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The Resource Wound healing : methods and protocols, edited by Luisa A. DiPietro and Aime L. Burns

Wound healing : methods and protocols, edited by Luisa A. DiPietro and Aime L. Burns

Wound healing : methods and protocols
Wound healing
Title remainder
methods and protocols
Statement of responsibility
edited by Luisa A. DiPietro and Aime L. Burns
During the past decade the significant progress made in cell and molecular biotechnology has led to exciting discoveries in the field of tissue repair, producing a wealth of information about the healing process. In Wound Healing: Methods and Protocols, expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. Useful for the study of many biological processes, including angiogenesis, epidermal differentiation and repair, acute inflammation, ECM synthesis, and remodeling, these powerful models illustrate biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure successful results. In many cases, several different approaches to a single process are examined, with sample results and analyses given to help users in selecting the approach most suited to the problem at hand. Comprehensive and enlightening, Wound Healing: Methods and Protocols offers both basic and clinical scientists a thought-provoking and highly practical collection of widely used model systems and methods for studying the injury repair process
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index present
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular medicine
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Wound healing : methods and protocols, edited by Luisa A. DiPietro and Aime L. Burns
Wound healing : methods and protocols, edited by Luisa A. DiPietro and Aime L. Burns
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Bibliography note
Includes bibliographical references and index
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online resource
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Excisional wound healing. An experimental approach / S. Frank and H. Kampfer -- Methods in reepithelialization. A porcine model of partial-thickness wounds / H.N. Paddock, G.S. Schultz and B.A. Mast -- Incisional wound healing. Model and analysis of wound breaking strength / R.L. Gamelli and L.K. He -- Animal models of ischemic wound healing. Toward an approximation of human chronic cutaneous ulcers in rabbit and rat / M. Sisco and T.A. Mustoe -- Corneal injury. A relatively pure model of stromal-epithelial interactions in wound healing / S.E. Wilson [and others] -- Subcutaneous sponge models / D.T. Efron and A. Barbul -- A mouse model of burn wounding and sepsis / J.M. Stevenson, R.L. Gamelli and R. Shankar -- A porcine burn model / A.J. Singer and S.A. McClain -- Wound healing in airways in vivo / S.R. White -- Murine models of intestinal anastomoses / D.L. Williams and I.W. Browder -- Murine model of peritoneal adhesion formation / A.E. Jahoda, M.K. Olson and E.J. Kovacs -- Methods for investigating fetal tissue repair / Z.M. Peled [and others] -- Growth of human blood vessels in severe combined immunodeficient mice. A new in vivo model system of angiogenesis / P.J. Polverini [and others] -- Tissue repair in models of diabetes mellitus. A review / D.G. Greenhalgh -- Wound healing studies in transgenic and knockout mice. A review / R. Grose and S. Werner -- Wound repair in aging. A review / M.J. Reed, T. Koike and P. Puolakkainen -- Specimen collection and analysis. Burn wounds / A. Kowal-Vern and B.A. Latenser -- Suction blister model of wound healing / V. Koivukangas and A. Oikarinen -- Implantable wound healing models and the determination of subcutaneous collagen deposition in expanded polytetrafluoroethylene implants / L.N. Jorgensen, S.M. Madsen and F. Gottrup -- The fibroblast-populated collagen lattice. A model of fibroblast collagen interactions in repair / H.P. Ehrlich -- A quantifiable in vitro model to assess effects of PAI-1 gene targeting on epithelial cell motility / K.M. Providence, L. Staiano-Coico and P.J. Higgins -- Human skin organ culture / I. Moll -- In vitro matrigel angiogenesis model / A.M. Szpaderska and L.A. DiPietro -- Quantification of wound angiogenesis / Q.E. Low and L.A. DiPietro -- In vivo matrigel migration and angiogenesis assays / K.M. Malinda -- Endothelial cell migration assay. A quantitative assay for prediction of in vivo biology / M.W. Lingen -- Analysis of collagen synthesis / R.F. Diegelmann -- Method for detection and quantitation of leukocytes during wound healing / I. Drugea and A.L. Burns -- Detection of reactive oxygen intermediate production by macrophages / J.E. Albina and J.S. Reichner -- Measurement of chemokines at the protein level in tissue / R.M. Strieter [and others] -- Methods of measuring oxygen in wounds / H.W. Hopf [and others] -- Isolation, culture, and characterization of human intestinal smooth muscle cells / M. Graham and A. Willey -- Use of high-throughput microarray membranes for cDNA analysis of cutaneous wound repair / N.S. Gibran and F.F. Isik -- Particle-mediated gene therapy of wounds / J.M. Davidson, S.A. Eming and J. Dasgupta
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1 online resource (xv, 467 pages)
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illustrations (some color).
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