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The Resource Two hybrid technologies : methods and protocols, edited by Bernhard Suter, Erich E. Wanker

Two hybrid technologies : methods and protocols, edited by Bernhard Suter, Erich E. Wanker

Label
Two hybrid technologies : methods and protocols
Title
Two hybrid technologies
Title remainder
methods and protocols
Statement of responsibility
edited by Bernhard Suter, Erich E. Wanker
Contributor
Editor
Subject
Genre
Language
eng
Summary
Protein-protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
812
Two hybrid technologies : methods and protocols, edited by Bernhard Suter, Erich E. Wanker
Label
Two hybrid technologies : methods and protocols, edited by Bernhard Suter, Erich E. Wanker
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-455-1
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical reference and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Matrix-based yeast two-hybrid screen strategies and comparison of systems -- Array-based yeast two-hybrid screens: A practical guide -- High-throughput yeast two-hybrid screening -- A stringent yeast two-hybrid matrix screening approach for protein-protein interaction discovery -- High-throughput yeast two-hybrid screening of complex cDNA libraries -- Virus-human cell interactomes -- Interactome mapping in malaria parasites: Challenges and opportunities -- Mapping interactomes with high coverage and efficiency using the shifted transversal design -- Assigning confidence scores to protein-protein interactions -- The integration and annotation of the human interactome in the UniHI database -- Gene-centered yeast one-hybrid assays -- One- plus two-hybrid system for the efficient selection of missense mutant alleles defective in protein-protein interactions -- Investigation of membrane protein interactions using the split-ubiquitin membrane yeast two-hybrid system -- Application of the split-protein sensor trp1 to protein interaction discovery in the yeast saccharomyces cerevisiae -- Tetracycline repressor-based mammalian two-hybrid systems -- The fluorescent two-hybrid (F2H) assay for direct analysis of protein-protein interactions in living cells -- ArrayMAPPIT: A screening platform for human protein interactome analysis -- MAPPIT as a high-throughput screening assay for modulators of protein-protein interactions in HIV and HCV -- Integrated measurement of split TEV and Cis-regulatory assays using EXT encoded reporter libraries
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617794551&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357733'}
Extent
1 online resource (xi, 329 pages)
File format
unknown
Form of item
online
Isbn
9781617794551
Lccn
2011940814
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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