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The Resource Transmembrane signaling protocols, edited by Hydar Ali, Bodduluri Haribabu

Transmembrane signaling protocols, edited by Hydar Ali, Bodduluri Haribabu

Transmembrane signaling protocols
Transmembrane signaling protocols
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edited by Hydar Ali, Bodduluri Haribabu
The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Transmembrane signaling protocols, edited by Hydar Ali, Bodduluri Haribabu
Transmembrane signaling protocols, edited by Hydar Ali, Bodduluri Haribabu
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Includes bibliographical references and index
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online resource
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  • Transmembrane signaling by G protein-coupled receptors / Louis M. Luttrell -- Crosstalk coregulation mechanisms of G protein-coupled receptors and receptor tyrosine kinases / Kanchana Nataranjan and Bradford C. Berk -- Protein-protein interactions in signaling cascades / Bruce J. Mayer -- Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells / Marcin Majka and Mariusz Z. Ratajczak -- Functional expression of CXCR4 in Saccharomyces cerevisiae in the development of powerful tools for pharmacological characterization of CXCR4 / Zi-xuan Wang, James R. Broach, and Stephen C. Peiper -- Characterization of constitutively active mutants of G protein-coupled receptors / Jean-Marc Navenot, Zi-xuan Wang, and Stephen C. Peiper -- G protein-coupled receptor dimerization and signaling / Mario Mellado [and others] -- Real-time analysis of G protein-coupled receptor signaling in live cells / Venkatakrishna R. Jala and Bodduluri Haribabu -- Isolation of membrane rafts and signaling complexes / Kathleen Boesze-Battaglia -- Methods for the study of signaling molecules in membrane lipid rafts and caveolae / Rennolds S. Ostrom and Paul A. Insel
  • Bioluminescence resonance energy transfer to monitor protein-protein interactions / Tarik Issad and Ralf Jockers -- Identification of interacting proteins using the yeast two-hybrid screen / Kelly L. Jordan-Sciutto and Marshall B. Montgomery -- Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays / Hyun Woo Lee, Jaewon Ko, and Eunjoon Kim -- Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins / Jaewon Ko and Eunjoon Kim -- Mammalian cell microinjection assay to study the function of rho family guanosine triphosphatases / Ritu Garg and Anne J. Ridley -- Affinity-based assay of Rho guanosine triphosphatase activation / Mary Stofega, Celine DerMardirossian, and Gary M. Bokoch -- Assay of phospholipase D activity in cell-free systems / Shankar S. Iyer and David J. Kusner -- Reconstitution system based on cytosol-depleted cells to study the regulation of phospholipase D / Amanda Fensome-Green and Shamshad Cockcroft -- Analysis of global gene expression profiles activated by chemoattractant receptors / Fernando O. Martinez and Massimo Locati -- Genetic reconstitution of bone marrow for the study of signal transduction ex vivo / Martha S. Jordan -- Proteomic analysis of human neutrophils / George Lominadze [and others]
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2nd ed.
1 online resource (xi, 364 pages)
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