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The Resource Transgenic maize : methods and protocols, edited by M. Paul Scott

Transgenic maize : methods and protocols, edited by M. Paul Scott

Label
Transgenic maize : methods and protocols
Title
Transgenic maize
Title remainder
methods and protocols
Statement of responsibility
edited by M. Paul Scott
Contributor
Subject
Genre
Language
eng
Summary
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs
Member of
Cataloging source
COO
Illustrations
  • illustrations
  • plates
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
526
Transgenic maize : methods and protocols, edited by M. Paul Scott
Label
Transgenic maize : methods and protocols, edited by M. Paul Scott
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-59745-494-0
Publication
Related Contributor
Related Location
Related Agents
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Design of gene constructs for transgenic maize / D. Liu -- Transient expression of GFP in immature seed tissues / C.T. Shepherd -- Biolistic gun-mediated maize genetic transformation / K. Wang and B. Frame -- Agrobacterium-mediated maize transformation: Immature embryos versus callus / V. Sidorov and D. Duncan -- Whiskers-mediated maize transformation / J.F. Petolino and N.L. Arnold -- Methods for generation and analysis of fluorescent protein-tagged maize lines / A. Mohanty [and others] -- Use of transgene-induced RNAi to regulate endogenous gene expression / K. McGinnis -- Plasmid rescue: Recovery of flanking genomic sequences from transgenic transposon insertion sites / G.-L. Nan and V. Walbot -- Nonradioactive genomic DNA blots for detection of low abundant sequences in transgenic maize / G.-L. Nan and V. Walbot -- Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds / M.P. Scott -- Determination of transgene copy number by real-time quantitative PCR / C.T. Shepherd, A.N. Moran Lauter and M.P. Scott -- Herbicide resistance screening assay / J.M. Peterson -- Characterizing transgene inheritance / E.H. Bicar -- Backcross breeding / K.E. Vogel
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781597454940&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357347'}
Extent
1 online resource (ix, 180 pages, [8] pages of color plates)
Form of item
online
Isbn
9781597454940
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

Library Locations

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      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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