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The Resource Site-specific recombinases : methods and protocols, edited by Nikolai Eroshenko

Site-specific recombinases : methods and protocols, edited by Nikolai Eroshenko

Site-specific recombinases : methods and protocols
Site-specific recombinases
Title remainder
methods and protocols
Statement of responsibility
edited by Nikolai Eroshenko
This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field
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index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
v. 1642
Site-specific recombinases : methods and protocols, edited by Nikolai Eroshenko
Site-specific recombinases : methods and protocols, edited by Nikolai Eroshenko
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Bibliography note
Includes bibliographical references and index
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online resource
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  • Use of the DICE (dual integrase cassette exchange) system
  • Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos
  • Ligand-controlled site-specific recombination in zebrafish
  • Avinash Chekuru, Veronika Kuscha, Stefan Hans, and Michael Brand
  • Injection-based delivery of cell-permeable peptide-tagged cre
  • Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, and Michael T. Chin
  • Viral delivery of GFP-dependent recombinases to the mouse brain
  • Jonathan C.Y. Tang, Stephanie Rudolph, and Constance L. Cepko
  • Recombinase-mediated cassette exchange using adenoviral vectors
  • Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell
  • Generating genetically modified mice : a decision guide
  • Marker removal in transgenic plants using cre recombinase delivered with potato virus X
  • Lilya Kopertekh and Joachim Schiemann
  • Nanoparticle-mediated recombinase delivery into maize
  • Susana Martin-Ortigosa, Brian G. Trewyn, and Kan Wang
  • Immunohistochemical procedures for characterizing the retinal expression patterns of cre driver mouse lines
  • Qi Lu and Zhuo-Hua Pan
  • FLPing genes on and off in Drosophila
  • Bonnie M. Weasner, Jinjin Zhu, and Justin P. Kumar
  • Imaging neural architecture in brainbow samples
  • Douglas H. Roossien and Dawen Cai
  • Ivo J. Huijbers
  • Purification and in vitro characterization of zinc finger recombinases
  • Femi J. Olorunniji, Susan J. Rosser, and W. Marshall Stark
  • Preparing mate-paired illumina libraries using cre recombinase
  • Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, and Feng Chen
  • Preparing fosmid mate-paired libraries using Cre-LoxP recombination
  • Ze Peng, Jeff L. Froula, and Jan-Fang Cheng
  • Using purified tyrosine site-specific recombinases in vitro to rapidly construct and diversify metabolic pathways
  • Wei Liu, Laura R. Tuck, Jon Marles Wright, and Yizhi Cai
  • Multipart DNA assembly using site-specific recombinases from the large serine integrase family
  • Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C.M. Smith, W. Marshall Stark, and Sean D. Colloms
  • Direct generation of conditional alleles using CRISPR/Cas9 in mouse zygotes
  • Production of minicircle DNA vectors using site-specific recombinases
  • Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani, and Mohammad Hossein Nasr-Esfahani
  • Colin E.J. Pritchard, Lona J. Kroese, and Ivo J. Huijbers
  • Building cre knockin rat lines using CRISPR/Cas9
  • Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang
  • Dual recombinase-mediated cassette exchange by tyrosine site-specific recombinases
  • Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov
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1 online resource (xi, 342 pages)
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illustrations (some color).
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      110 8th St, Troy, NY, 12180, US
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