Coverart for item
The Resource Single-stranded DNA binding proteins : methods and protocols, edited by James L. Keck

Single-stranded DNA binding proteins : methods and protocols, edited by James L. Keck

Label
Single-stranded DNA binding proteins : methods and protocols
Title
Single-stranded DNA binding proteins
Title remainder
methods and protocols
Statement of responsibility
edited by James L. Keck
Contributor
Subject
Genre
Language
eng
Summary
The genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information. However, DNA unwinding comes with inherent risks to genome stability. To help mediate these risks, bacterial, archael, and eukaryotic cells have evolved protective ssDNA-binding proteins (SSBs) that bind ssDNA with high affinity and specificity. SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes. Single-Stranded DNA Binding Proteins: Methods and Protocols assembles methods developed for examining the fundamental properties of SSBs and for exploiting the biochemical functions of SSBs for their use as in vitro and in vivo reagents. Clearly and concisely organized, the volume opens with an introduction to the structures and functions of SSBs, followed protocols for studying SSB/DNA complexes, methods for studying SSB/heterologous protein complexes, protocols for interrogating post-translational modifications of SSBs, and concludes with uses of fluorescently-labeled SSBs for in vitro and in vivo studies of genome maintenance processes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Single-Stranded DNA Binding Proteins: Methods and Protocols provides a rich introduction for investigators who are interested in this fascinating family of DNA-binding proteins
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
922
Single-stranded DNA binding proteins : methods and protocols, edited by James L. Keck
Label
Single-stranded DNA binding proteins : methods and protocols, edited by James L. Keck
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-62703-032-8
Publication
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Related Agents
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Single-molecule analysis of SSB dynamics on single-stranded DNA
  • Ruobo Zhou and Taekjip Ha
  • Sample preparation methods to analyze DNA-induced structural changes in replication protein A
  • Chris A. Brosey, Susan E. Tsutakawa, and Walter J. Chazin
  • Structural studies of SSB interaction with RecO
  • Mikhail Ryzhikov and Sergey Korolev
  • Investigation of protein-protein interactions of single-stranded DNA-binding proteins by analytical ultracentrifugation
  • Natalie Naue and Ute Curth
  • Ammonium sulfate co-precipitation of SSB and interacting proteins
  • Aimee H. Marceau
  • Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair
  • Analyzing Interactions Between SSB and proteins by the use of fluorescence anisotropy
  • Duo Lu
  • Far western blotting as a rapid and efficient method for detecting interactions between DNA replication and DNA repair proteins
  • Brian W. Walsh, Justin S. Lenhart, Jeremy W. Schroeder, and Lyle A. Simmons
  • Methods for analysis of SSB-protein interactions by SPR
  • Asher N. Page and Nicholas P. George
  • Use of native gels to measure protein binding to SSB
  • Jin Inoue and Tsutomu Mikawa
  • Identification of small molecules that disrupt SSB-protein interactions using a high-throughput screen
  • Douglas A. Bernstein
  • Aimee H. Marceau
  • Detection of posttranslational modifications of replication protein A
  • Cathy S. Hass, Ran Chen, and Marc S. Wold
  • Detecting posttranslational modifications of bacterial SSB proteins
  • Dusica Vujaklija and Boris Macek
  • Fluorescent SSB as a reagentless biosensor for single-stranded DNA
  • Katy Hedgethorne and Martin R. Webb
  • Fluorescent single-stranded DNA-binding proteins enable in vitro and in vivo studies
  • Piero R. Bianco, Adam J. Stanenas, Juan Liu, and Christopher S. Cohan
  • Use of fluorescently tagged SSB proteins in in vivo localization experiments
  • Rodrigo Reyes-Lamothe
  • Structural diversity based on variability in quaternary association. a case study involving eubacterial and related SSBs
  • S.M. Arif and M. Vijayan
  • SSB binding to ssDNA using isothermal titration calorimetry
  • Alexander G. Kozlov and Timothy M. Lohman
  • SSB-DNA binding monitored by fluorescence intensity and anisotropy
  • Alexander G. Kozlov, Roberto Galletto, and Timothy M. Lohman
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781627030328&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357836'}
Extent
1 online resource (x, 259 pages)
File format
unknown
Form of item
online
Isbn
9781627030328
Lccn
2012945406
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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