Borrow it
Coverart for item
The Resource Single molecule analysis : methods and protocols, edited by Erwin J.G. Peterman and Gijs J.L. Wuite

Single molecule analysis : methods and protocols, edited by Erwin J.G. Peterman and Gijs J.L. Wuite

Contributor
Summary
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology
Language
eng
Work
Publication
Extent
1 online resource (x, 317 pages).
Note
Includes index
Contents
  • Brief introduction to single-molecule fluorescence methods
  • Siet M.J.L. van den Wildenberg, Bram Prevo, and Erwin J.G. Peterman
  • Fluorescent labeling of proteins
  • Mauro Modesti
  • Fluorescence imaging of single kinesin motors on immobilized microtubules
  • Till Korten [and others]
  • Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging
  • Julie S. Biteen, Lucy Shapiro, and W.E. Moerner
  • Fluorescence microscopy of nanochannel-confined DNA
  • Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt
  • Introduction to optical tweezers : background, system designs, and commercial solutions
  • Fluorescence correlation spectroscopy
  • Patrick Ferrand, Jerome Wenger, and Herve Rigneault
  • Introduction to atomic force microscopy
  • Pedro J. de Pablo
  • Sample preparation for SFM imaging of DNA, proteins, and DNA-protein complexes
  • Dejan Ristic, Humberto Sanchez, and Claire Wyman
  • Single-molecule protein unfolding and refolding using atomic force microscopy
  • Thomas Bornschlogl and Matthias Rief
  • How to perform a nanoindentation experiment on a virus
  • Wouter H. Roos
  • Joost van Mameren, Gijs J.L. Wuite, and Iddo Heller
  • Magnetic tweezers for single-molecule manipulation
  • Yeonee Seol and Keir C. Neuman
  • Probing DNA topology using tethered particle motion
  • David Dunlap [and others]
  • Optical trapping and unfolding of RNA
  • Katherine H. White and Koen Visscher
  • DNA unzipping and force measurements with a dual optical trap
  • Ismaıl Cisse, Pierre Mangeol, and Ulrich Bockelmann
  • Probing the force generation and stepping behavior of cytoplasmic dynein
  • Arne Gennerich and Samara L. Reck-Peterson
Isbn
9781617792823
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-282-3
Instance
Label
Single molecule analysis : methods and protocols
Title
Single molecule analysis
Title remainder
methods and protocols
Statement of responsibility
edited by Erwin J.G. Peterman and Gijs J.L. Wuite
Contributor
Subject
Genre
Language
eng
Summary
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology
Member of
Cataloging source
GW5XE
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
783
Single molecule analysis : methods and protocols, edited by Erwin J.G. Peterman and Gijs J.L. Wuite
Label
Single molecule analysis : methods and protocols, edited by Erwin J.G. Peterman and Gijs J.L. Wuite
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-282-3
Publication
Note
Includes index
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Brief introduction to single-molecule fluorescence methods
  • Siet M.J.L. van den Wildenberg, Bram Prevo, and Erwin J.G. Peterman
  • Fluorescent labeling of proteins
  • Mauro Modesti
  • Fluorescence imaging of single kinesin motors on immobilized microtubules
  • Till Korten [and others]
  • Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging
  • Julie S. Biteen, Lucy Shapiro, and W.E. Moerner
  • Fluorescence microscopy of nanochannel-confined DNA
  • Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt
  • Introduction to optical tweezers : background, system designs, and commercial solutions
  • Fluorescence correlation spectroscopy
  • Patrick Ferrand, Jerome Wenger, and Herve Rigneault
  • Introduction to atomic force microscopy
  • Pedro J. de Pablo
  • Sample preparation for SFM imaging of DNA, proteins, and DNA-protein complexes
  • Dejan Ristic, Humberto Sanchez, and Claire Wyman
  • Single-molecule protein unfolding and refolding using atomic force microscopy
  • Thomas Bornschlogl and Matthias Rief
  • How to perform a nanoindentation experiment on a virus
  • Wouter H. Roos
  • Joost van Mameren, Gijs J.L. Wuite, and Iddo Heller
  • Magnetic tweezers for single-molecule manipulation
  • Yeonee Seol and Keir C. Neuman
  • Probing DNA topology using tethered particle motion
  • David Dunlap [and others]
  • Optical trapping and unfolding of RNA
  • Katherine H. White and Koen Visscher
  • DNA unzipping and force measurements with a dual optical trap
  • Ismaıl Cisse, Pierre Mangeol, and Ulrich Bockelmann
  • Probing the force generation and stepping behavior of cytoplasmic dynein
  • Arne Gennerich and Samara L. Reck-Peterson
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617792823&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357689'}
Extent
1 online resource (x, 317 pages).
Form of item
online
Isbn
9781617792823
Lccn
2011935030
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote

Subject

Genre

Member of

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577

Library Links

Structured data from the Bibframe namespace is licensed under the Creative Commons Attribution 4.0 International License by Rensselaer Libraries. Additional terms may apply to data associated with third party namespaces.
Processing Feedback ...