Coverart for item
The Resource SiRNA design : methods and protocols, edited by Debra J. Taxman

SiRNA design : methods and protocols, edited by Debra J. Taxman

Label
SiRNA design : methods and protocols
Title
SiRNA design
Title remainder
methods and protocols
Statement of responsibility
edited by Debra J. Taxman
Contributor
Subject
Language
eng
Summary
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design
Member of
Cataloging source
SCB
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
942
SiRNA design : methods and protocols, edited by Debra J. Taxman
Label
SiRNA design : methods and protocols, edited by Debra J. Taxman
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-62703-119-6
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Engineering small interfering RNAs by strategic chemical modification
  • Jesper B. Bramsen and Jørgen Kjems
  • Design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs
  • Narendra Vaish and Pinky Agarwal
  • Design, preparation and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells
  • Chanil Chang [and others]
  • Design of nuclease-resistant fork-like small interfering RNA (fsiRNA)
  • Elena L. Chernolovskaya and Marina A. Zenkova
  • Designing dual-targeting siRNA duplexes having two active strands that combine siRNA and MicroRNA-like targeting
  • Pal Sætrom
  • What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs
  • Strategies for designing and Validating immunostimulatory siRNAs
  • Michael P. Gantier
  • Designing efficient and specific endoribonuclease-prepared siRNAs
  • Vineeth Surendranath [and others]
  • Short hairpin RNA-mediated gene silencing
  • Luke S. Lambeth and Craig A. Smith
  • Design of lentivirally expressed siRNAs
  • Ying Poi Liu and Ben Berkhout
  • Bifunctional short hairpin RNA (bi-shRNA) : design and pathway to clinical application
  • Donald D. Rao [and others]
  • Olga Matveeva
  • Design and chemical modification of synthetic short shRNAs as potent RNAi triggers
  • Anne Dallas and Brian H. Johnston
  • Production and application of long dsRNA in mammalian cells
  • Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda
  • Design of RNAi reagents for invertebrate model organisms and human disease vectors
  • Thomas Horn and Michael Boutros
  • Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase
  • Hiromitsu Tanaka
  • Designing effective amiRNA and multimeric amiRNA against plant viruses
  • Muhammad Fahim and Philip J. Larkin
  • Methods for selecting effective siRNA target sequences using a variety of statistical and analytical techniques
  • Downregulation of plant genes with miRNA-induced gene silencing
  • Felipe Fenselau de Felippes
  • Shigeru Takasaki
  • Designing functional siRNA with reduced off-target effects
  • Yuki Naito and Kumiko Ui-Tei
  • Design and screening of siRNAs against highly structured RNA targets
  • Neda Nasheri, John Paul Pezacki, and Selena M. Sagan
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{'f': 'http://opac.lib.rpi.edu/record=b3648651'}
Extent
1 online resource (xiii, 392 pages)
Form of item
online
Isbn
9781627031196
Lccn
2012947406
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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