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The Resource Ribozymes : methods and protocols, edited by Jörg S. Hartig

Ribozymes : methods and protocols, edited by Jörg S. Hartig

Label
Ribozymes : methods and protocols
Title
Ribozymes
Title remainder
methods and protocols
Statement of responsibility
edited by Jörg S. Hartig
Contributor
Subject
Genre
Language
eng
Summary
Over the past decade, there have been a variety of fundamental insights into ribozymes which have changed the perspective on RNA catalysis. In, Ribozymes: Methods and Protocols, researchers in the field detail many of the protocols and techniques which are now commonly used to study individual ribozymes and the characterization of ribozymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ribozymes: Methods and Protocols seeks to aid scientists in further understanding the catalytically active RNAs
Member of
Cataloging source
GW5XE
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
dictionaries
Series statement
Methods in molecular biology,
Series volume
848
Ribozymes : methods and protocols, edited by Jörg S. Hartig
Label
Ribozymes : methods and protocols, edited by Jörg S. Hartig
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-545-9
Publication
Note
Includes index
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction -- Characterization of hammerhead ribozyme reactions -- Mechanistic analysis of the hepatitis delta virus (HDV) ribozyme: Methods for RNA preparation, structure mapping, solvent isotope effects, and co-transcriptional cleavage -- Kinetic characterization of hairpin ribozyme variants -- Characterization of RNase p rna activity -- Group I intron ribozymes -- Kinetic characterization of group II intron folding and splicing -- Mechanism and distribution of glmS ribozymes -- Structure-based search and in vitro analysis of self-cleaving ribozymes -- Discovery of rna motifs using a computational pipeline that allows insertions in paired regions and filtering of candidate sequences -- Crystallographic analysis of small ribozymes and riboswitches -- Functional dynamics of RNA ribozymes studied by NMR spectroscopy -- Deoxyribozyme-based, semisynthetic access to stable peptidyl-trnas exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide -- Probing functions of the ribosomal peptidyl transferase center by nucleotide analog interference -- Single molecule fret characterization of large ribozyme folding -- Metal ion-RNA interactions studied via multinuclear NMR -- Analysis of catalytic RNA structure and function by nucleotide analog interference mapping -- In vitro selection of metal ion-selective dnazymes -- Selecting allosteric ribozymes -- Screening effective target sites on mRNA: A ribozyme library approach -- A computational approach to predict suitable target sites for trans-acting minimal hammerhead ribozymes -- Targeting mRNAs by engineered sequence-specific RNase p ribozymes -- Target-induced SOFA-HDV ribozyme -- Ribozyme-mediated trans insertion-splicing into target RNAs -- Developing fluorogenic RNA-cleaving dnazymes for biosensing applications -- Development of trainable deoxyribozyme-based game playing automaton -- Rational design and tuning of ribozyme-based devices -- In vivo screening of ligand-dependent hammerhead ribozymes -- Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617795442&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357744'}
Extent
1 online resource (xiv, 480 pages).
File format
unknown
Form of item
online
Isbn
9781617795442
Lccn
2012930264
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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