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The Resource Recombinant protein production in yeast : methods and protocols, edited by Roslyn M. Bill

Recombinant protein production in yeast : methods and protocols, edited by Roslyn M. Bill

Contributor
Summary
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host
Language
eng
Work
Publication
Extent
1 online resource (xii, 248 pages).
Note
Includes index
Contents
  • Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill
  • Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby [and others]
  • Preparation of Pichia pastoris expression plasmids / Christel Logez [and others]
  • Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim
  • Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk
  • Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby
  • Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh [and others]
  • Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim
  • The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill
  • Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare
  • The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora [and others]
  • Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji
  • Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji
  • Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji
  • Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby
  • Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim
  • Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh [and others]
  • Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora
  • Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby
Isbn
9781617797699
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-770-5
Instance
Label
Recombinant protein production in yeast : methods and protocols
Title
Recombinant protein production in yeast
Title remainder
methods and protocols
Statement of responsibility
edited by Roslyn M. Bill
Contributor
Subject
Genre
Language
eng
Summary
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host
Member of
Cataloging source
GW5XE
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
866
Recombinant protein production in yeast : methods and protocols, edited by Roslyn M. Bill
Label
Recombinant protein production in yeast : methods and protocols, edited by Roslyn M. Bill
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-770-5
Publication
Note
Includes index
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby [and others] -- Preparation of Pichia pastoris expression plasmids / Christel Logez [and others] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora [and others] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby
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http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357763'}
Extent
1 online resource (xii, 248 pages).
File format
unknown
Form of item
online
Isbn
9781617797699
Lccn
2012933363
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
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