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The Resource Receptor binding techniques, edited by Anthony P. Davenport

Receptor binding techniques, edited by Anthony P. Davenport

Label
Receptor binding techniques
Title
Receptor binding techniques
Statement of responsibility
edited by Anthony P. Davenport
Contributor
Subject
Genre
Language
eng
Summary
A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel 'biased agonists'. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
v. 897
Receptor binding techniques, edited by Anthony P. Davenport
Label
Receptor binding techniques, edited by Anthony P. Davenport
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-909-9
Publication
Note
Previous edition: 2005
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Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Jenny Berry, Molly Price-Jones, and Barbara Killian
  • Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands
  • Craig J. Daly, Ingela Parmryd, and John C. McGrath
  • Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation
  • Laura E. Kilpatrick and Nicholas D. Holliday
  • Live cell imaging of G protein-coupled receptors
  • Anke Teichmann [and others]
  • Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays
  • Daniel L. Bassoni [and others]
  • Measurements of [beta]-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation
  • Daniel L. Bassoni [and others]
  • Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography
  • Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport
  • Dynamic in vivo imaging of receptors in small animals using positron emission tomography
  • Peter Johnstrom [and others]
  • Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy
  • Anthony P. Davenport and Rhoda E. Kuc
  • Detection of mRNA encoding receptors by in situ and northern hybridization
  • Alessandra P. Princivalle [and others]
  • Receptor databases and computational websites for ligand binding
  • Brinda K. Rana, Philip E. Bourne, and Paul A. Insel
  • How to use the IUPHAR receptor database to navigate pharmacological data
  • Chidochangu P. Mpamhanga [and others]
  • Radioligand binding assays and their analysis
  • Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport
  • Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617799099&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357786'}
Edition
3rd ed.
Extent
1 online resource (xii, 309 pages)
File format
unknown
Form of item
online
Isbn
9781617799099
Lccn
2012938879
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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