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The Resource RNAi and small regulatory RNAs in stem cells : methods and protocols, edited by Baohong Zhang

RNAi and small regulatory RNAs in stem cells : methods and protocols, edited by Baohong Zhang

RNAi and small regulatory RNAs in stem cells : methods and protocols
RNAi and small regulatory RNAs in stem cells
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methods and protocols
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edited by Baohong Zhang
This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature
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Methods in molecular biology,
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volume 1622
RNAi and small regulatory RNAs in stem cells : methods and protocols, edited by Baohong Zhang
RNAi and small regulatory RNAs in stem cells : methods and protocols, edited by Baohong Zhang
Includes index
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Includes bibliographical references
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  • High-efficiency transfection of glioblastoma cells and a simple spheroid migration assay
  • Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Löhr
  • Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells
  • Xiaoxing Cheng and Rui Jian
  • Efficient depletion of essential gene products for loss-of-function studies in embryonic stem cells
  • Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, and Matthieu Gérard
  • Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8
  • Takayuki Manabe and Akio Wanaka
  • Recessive genetic screen for components of the RNA interference pathway performed in mouse embryonic stem cells
  • Melanie I. Trombly and Xiaozhong Wang
  • RNAi technique in stem cell research : current status and future perspectives
  • RNAi knockdown of Ape1 gene in the differentiation of mouse embryonic stem cells
  • Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
  • Efficient transfection method for differentiation and cell proliferation of mouse embryonic stem cells
  • Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
  • Implanting glioblastoma spheroids into rat brains and monitoring tumor growth by MRI volumetry
  • Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
  • RNAi-based techniques for the analysis of gene function in Drosophila germline stem cells
  • Amelia J. Blake, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
  • In vivo RNAi in the Drosophila follicular epithelium : analysis of stem cell maintenance, proliferation, and differentiation
  • Veit Riechmann
  • Gang-Ming Zou
  • Phenotype-based RNAi screening for Ras-ERK/MAPK signaling-associated stem cell regulators in C. elegans
  • Myon-Hee Lee and Dong Suk Yoon
  • Engineering human mesenchymal stem cells to release adenosine using miRNA technology
  • Gaoying Ren and Detlev Boison
  • Efficient gene knockdowns in mouse embryonic stem cells using MicroRNA-based shRNAs
  • Jianlong Wang
  • Using quantitative real-time PCR to detect MicroRNA expression profile during embryonic stem cell differentiation
  • Xiaoping Pan, Alexander K. Murashov, Edmund J. Stellwag, and Baohong Zhang
  • Genetic tools for self-organizing culture of mouse embryonic stem cells via small regulatory RNA-mediated technologies, CRISPR/Cas9, and inducible RNAi
  • Nozomu Takata, Eriko Sakakura, Tetsushi Sakuma, and Takashi Yamamoto
  • RNAi and MicroRNA-mediated gene regulation in stem sells
  • CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells
  • Yinghua Wang, Yifu Ding, and Jinsong Li
  • Alexander K. Murashov
  • Forward RNAi screens in human hematopoietic stem cells
  • Roman Galeev, Christine Karlsson, Aurélie Baudet, and Jonas Larsson
  • Silencing of ATP11B by RNAi-induced changes in neural stem cell morphology
  • Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, and Tieqiao Wen
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1 online resource (xi, 311 pages)
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