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The Resource Protocols for nucleic acid analysis by nonradioactive probes, edited by Peter G. Isaac

Protocols for nucleic acid analysis by nonradioactive probes, edited by Peter G. Isaac

Contributor
Summary
This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification
Language
eng
Work
Publication
Extent
1 online resource (xii, 268 pages)
Note
Source of title; title from title screen
Contents
  • About nonradioactive nucleic acid detection
  • Isolation of DNA from plants
  • Isolation of high-molecular-weight DNA from animal cells
  • Restriction enzyme digestion, gel electrophoresis and vacuum blotting of DNA to nylon membranes
  • Isolation of plant RNA
  • Isolation of total and polyA RNA from animal cells
  • Preparation of RNA gel blots
  • Preparation of RNA dot blots
  • Isolation of plasmids for the preparation of probes
  • Production of hybridization probes by the PCR utilizing digoxigenin-modified nucleotides
  • Production of DNA hybridization probes with digoxigenin-modified nucleotides by random hexanucleotide priming
  • Digoxigenin labeling of RNA transcripts from multi- and single-locus DNA minisatellite probes
  • Labeling of double-stranded DNA probes with biotin
  • Preparation of horseradish peroxidase-labeled probes
  • Random prime labeling of DNA probes with fluorescein-11-dUTP
  • Labeling of oligonucleotides with fluorescein
  • Hybridization of digoxigenin-labeled probes to southern blots and detection by chemiluminescence
  • Southern blot hybridization of digoxigenin-labeled RNA minisatellite probes and color detection
  • Hybridization and detection of digoxigenin probes on RNA blots
  • Hybridization of horseradish peroxidase-labeled probes and detection by enhanced chemiluminescence
  • Hybridization and detection of fluorescein-labeled DNA probes using enhanced chemiluminescence
  • Hybridization of fluorescein-labeled oligonucleotide probes and enhanced chemiluminescence detection
  • Preparation of chromosome spreads by root-tip meristem dissection for in situ hybridization with biotin-labeled probes
  • Enzymatic treatment of plant material to spread chromosomes for in situ hybridization
  • Use of biotin-labeled probes on plant chromosomes
  • Direct fluorochrome-labeled DNA probes for direct fluorescent in situ hybridization to chromosomes
  • Detection of dioxigenin-labeled DNA probes hybridized to plant chromosomes in situ
  • Preparation of tissue sections and slides for mRNA hybridization
  • Detecting mRNA in tissue sections with digoxigenin-labeled probes
  • Detection of mRNA in whole mounts of mouse embryos using digoxigenin riboprobes
  • PACE (Probe Assay-Chemiluminescence Enhanced) : a magnetic bead assay for noncultural diagnosis of gonorrhea
  • Detection of foodborne pathogens using DNA probes and a dipstick format
  • Analysis of gene sequences by hybridization of PCR-amplified DNA to covalently bound oligonucleotide probes : the reverse dot blot method
  • RAPD assay: a novel technique for genetic diagnostics
  • Nonradioactive oligonucleotide probes for detecting products of the ligase chain reaction
  • Nucleic acid sequence-based amplification (NASBA)
Isbn
9781592595150
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/089603254X
Instance
Label
Protocols for nucleic acid analysis by nonradioactive probes
Title
Protocols for nucleic acid analysis by nonradioactive probes
Statement of responsibility
edited by Peter G. Isaac
Title variation
Nonradioactive probes
Contributor
Subject
Language
eng
Summary
This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification
Member of
Cataloging source
YUS
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 28
Protocols for nucleic acid analysis by nonradioactive probes, edited by Peter G. Isaac
Label
Protocols for nucleic acid analysis by nonradioactive probes, edited by Peter G. Isaac
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/089603254X
Publication
Note
Source of title; title from title screen
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
About nonradioactive nucleic acid detection -- Isolation of DNA from plants -- Isolation of high-molecular-weight DNA from animal cells -- Restriction enzyme digestion, gel electrophoresis and vacuum blotting of DNA to nylon membranes -- Isolation of plant RNA -- Isolation of total and polyA RNA from animal cells -- Preparation of RNA gel blots -- Preparation of RNA dot blots -- Isolation of plasmids for the preparation of probes -- Production of hybridization probes by the PCR utilizing digoxigenin-modified nucleotides -- Production of DNA hybridization probes with digoxigenin-modified nucleotides by random hexanucleotide priming -- Digoxigenin labeling of RNA transcripts from multi- and single-locus DNA minisatellite probes -- Labeling of double-stranded DNA probes with biotin -- Preparation of horseradish peroxidase-labeled probes -- Random prime labeling of DNA probes with fluorescein-11-dUTP -- Labeling of oligonucleotides with fluorescein -- Hybridization of digoxigenin-labeled probes to southern blots and detection by chemiluminescence -- Southern blot hybridization of digoxigenin-labeled RNA minisatellite probes and color detection -- Hybridization and detection of digoxigenin probes on RNA blots -- Hybridization of horseradish peroxidase-labeled probes and detection by enhanced chemiluminescence -- Hybridization and detection of fluorescein-labeled DNA probes using enhanced chemiluminescence -- Hybridization of fluorescein-labeled oligonucleotide probes and enhanced chemiluminescence detection -- Preparation of chromosome spreads by root-tip meristem dissection for in situ hybridization with biotin-labeled probes -- Enzymatic treatment of plant material to spread chromosomes for in situ hybridization -- Use of biotin-labeled probes on plant chromosomes -- Direct fluorochrome-labeled DNA probes for direct fluorescent in situ hybridization to chromosomes -- Detection of dioxigenin-labeled DNA probes hybridized to plant chromosomes in situ -- Preparation of tissue sections and slides for mRNA hybridization -- Detecting mRNA in tissue sections with digoxigenin-labeled probes -- Detection of mRNA in whole mounts of mouse embryos using digoxigenin riboprobes -- PACE (Probe Assay-Chemiluminescence Enhanced) : a magnetic bead assay for noncultural diagnosis of gonorrhea -- Detection of foodborne pathogens using DNA probes and a dipstick format -- Analysis of gene sequences by hybridization of PCR-amplified DNA to covalently bound oligonucleotide probes : the reverse dot blot method -- RAPD assay: a novel technique for genetic diagnostics -- Nonradioactive oligonucleotide probes for detecting products of the ligase chain reaction -- Nucleic acid sequence-based amplification (NASBA)
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unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356822'}
Extent
1 online resource (xii, 268 pages)
File format
unknown
Form of item
online
Isbn
9781592595150
Lccn
93029390
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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