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The Resource Protocols for gene analysis, edited by Adrian J. Harwood

Protocols for gene analysis, edited by Adrian J. Harwood

Label
Protocols for gene analysis
Title
Protocols for gene analysis
Statement of responsibility
edited by Adrian J. Harwood
Contributor
Subject
Language
eng
Summary
This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts
Member of
Cataloging source
YNG
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 31
Protocols for gene analysis, edited by Adrian J. Harwood
Label
Protocols for gene analysis, edited by Adrian J. Harwood
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/0896032582
Publication
Related Contributor
Related Location
Related Agents
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Basic recombinant DNA techniques -- Transformation of bacteria by electroporation -- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector -- PCR cloning using T-vectors -- Thermal cycle dideoxy DNA sequencing -- In vitro mutagenesis -- Ordered deletions using exonuclease III -- Site-directed mutagenesis using a double-stranded DNA template -- Site-directed mutagenesis using a uracil-containing phagemid template -- Construction of linker-scanning mutations by oligonucleotide ligation -- Construction of linker scanning mutations using the polymerase chain reaction -- Localized random polymerase chain reaction mutagenesis -- Genomic structure -- Simultaneous isolation of RNA and DNA from tissues and cultured cells -- Physical mapping of the human genome by pulsed field gel electrophoresis -- Field inversion gel electrophoresis -- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes -- Nonradioactive oligonucleotide probe labeling -- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels -- Inverse polymerase chain reaction -- Sequence variations -- Use of silver staining to detect nucleic acids -- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP) -- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA -- TGGE in quantitative PCR of DNA and RNA -- PGK-PCR clonality assay (PPCA) -- Direct sequencing of PCR products -- Gene expression -- Use of riboprobes for the analysis of gene expression -- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization -- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay -- RNA polymerase II in vitro transcription system -- S1 mapping using single-stranded DNA probes -- Single primer-mediated polymerase chain reaction -- Protein-DNA interactions -- In vivo DNA footprinting by linear amplification -- DNA photofootprinting with Rh(phi)2bpy3 -- Gel retardation assay -- Southwestern assay -- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes -- Protein function -- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification -- Production of 35S-labeled proteins in E. coli and their use as molecular probes -- Preparation and ligand screening of a [lambda]g11 lysogen library
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592595181&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357242'}
Extent
1 online resource (xiv, 411 pages)
File format
one file format
Form of item
online
Isbn
9781592595181
Lccn
94002365
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote

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