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The Resource Protein phosphatase protocols, edited by John W. Ludlow

Protein phosphatase protocols, edited by John W. Ludlow

Label
Protein phosphatase protocols
Title
Protein phosphatase protocols
Statement of responsibility
edited by John W. Ludlow
Contributor
Subject
Genre
Language
eng
Summary
In Protein Phosphatase Protocols, John Ludlow assembles a collection of cutting-edge techniques for investigating the structure and function of protein serine/threonine phosphatases. These methods are designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Written by leading researchers with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators. The book also includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Protein Phosphatase Protocols offers researchers the single best collection of step-by-step laboratory protocols now available for these important enzymes. Because of their easy reproducibility, these methods will greatly facilitate research in the area, and thus are certain to become indispensable for all those working to unlock the structure and function of the protein serine/threonine phosphatases
Member of
Cataloging source
N$T
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • handbooks
Series statement
Methods in molecular biology
Series volume
v. 93
Protein phosphatase protocols, edited by John W. Ludlow
Label
Protein phosphatase protocols, edited by John W. Ludlow
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/0896034682
Publication
Related Contributor
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Related Agents
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Related Subjects
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Prokaryotic protein-serine/threonine phosphatases / Peter J. Kennelly -- Protein phosphatase type 1 and Type 2A assays / S. Derek Killilea, Qi Cheng, Zhi-Xin Wang -- Analyzing gene expression with the use of serine/threonine phosphatase inhibitors / Axel H. Schönthal -- Inhibitor-1, a regulator of protein phosphatase 1 function / John H. Connor, Hai Quan, Carey Oliver, Shirish Shenolikar -- I1 PP2A and I2 PP2A : two potent protein phosphatase 2A-specific inhibitor proteins / Mei Li, Zahi Damuni -- Control of PP1 activity through phosphorylation by cyclin-dependent kinases / Norbert Berndt -- Regulation of neuronal PP1 and PP2A during development / Elizabeth Collins, Alistair T. Sim -- PTPA regulating PP2A as a dual specificity phosphatase / Veerle Janssens, Christine Hoof, Wilfried Merlevede, Jozef Goris -- Microinjection and immunological methods in the analysis of type 1 and 2A protein phosphatases from mammalian cells / Patric Turowski, Ned J. Lamb -- Use of immunocomplexed substrate for detecting PP1 activity / John W. Ludlow, Deirdre A. Nelson, Nancy A. Krucher -- The biochemical identification and characterization of new species of protein phosphatase 1 / Monique Beullens, Willy Stalmans, Mathieu Bollen -- The relationship between insulin signaling and protein phosphatase 1 activation / Louis Ragolia, Najma Begum -- Analysis of the isoforms of protein phosphatase 1 (PP1) with polyclonal peptide antibodies / Margherita Tognarini, Emma Villa-Moruzzi -- Expression of mouse protein phosphatase 2C in Eschericia coli and COS 7 cells / Takayasu Kobayashi, Kazuyuki Kusuda, Motoko Ohnishi, Naoki Chida, Shinri Tamura -- Expression of functional protein phosphatase 1 catalytic subunit in E. coli / Mariam Dohadwala, Norbert Berndt -- Protein phosphatase 2A and protein phosphatase X genes in Arabidopsis thaliana / Gemma Pujol, Albert Ferrer, Joaquin Ariño -- Separation of protein phosphatase Type 2C isozymes by chromatography on blue sepharose / Susanne Klumpp, Dagmar Selke -- Chromatographic isolation of PP2A from Limulus lateral eyes : conventional and small scale methods / Samuel C. Edwards, Travis B. Dyke, Timothy H. Dyke, David L. Brautigan -- Purification and assay of the Ptc1/Tpd1 protein phosphatase 2C from the yeast Saccharomyces cerevisiae / Matthew K. Robinson, Eric M. Phizicky -- Molecular cloning of protein phosphatase type 2C isoforms from retinal cDNA / Dagmar Selke, Susanne Klumpp, Benjamin Kaupp, Arnd Baumann -- Analysis of protein interactions between protein phosphatase 1 and noncatalytic subunits using the yeast two-hybrid assay / Nadja T. Ramaswamy, Brian K. Dalley, John F. Cannon -- Identifying protein phosphatase 2A interacting proteins using the yeast two-hybrid method / Brent McCright, David M. Virshup -- Protein phosphatase 2A regulatory subunits : cDNA cloning and analysis of mRNA expression / Julie A. Zaucha, Ryan S. Westphal, Brian E. Wadzinski -- Synthetic lethal screening in protein phosphatase pathways / Jianhong Zheng, John F. Cannon -- The search for the biological function of novel yeast Ser/Thr phosphatases / Joaquin Ariño, Francesc Posas, Josep Clotet
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Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356671'}
Extent
1 online resource (xiii, 316 pages)
Form of item
online
Isbn
9780896034686
Lccn
98015957
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

Library Locations

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