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The Resource Protein electrophoresis : methods and protocols, edited by Biji T. Kurien and R. Hal Scofield

Protein electrophoresis : methods and protocols, edited by Biji T. Kurien and R. Hal Scofield

Protein electrophoresis : methods and protocols
Protein electrophoresis
Title remainder
methods and protocols
Statement of responsibility
edited by Biji T. Kurien and R. Hal Scofield
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology
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v. 869
Protein electrophoresis : methods and protocols, edited by Biji T. Kurien and R. Hal Scofield
Protein electrophoresis : methods and protocols, edited by Biji T. Kurien and R. Hal Scofield
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Includes bibliographical references and index
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online resource
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  • Mandy Peach, Noelle Marsh, and Daniel J. MacPhee
  • Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni
  • Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate schiff-base stain
  • Padmaja Mehta-D'souza
  • Spicy SDS-PAGE gels : curcumin/turmeric as an environment-friendly protein stain
  • Biji T. Kurien, Yaser Dorri, and R. Hal Scofield
  • In-gel staining of proteins in native polyacrylamide gel electrophoresis using meso-tetrakis(4-sulfonatophenyl) porphyrin
  • K. Divakar, G. Nandhini Devi, and Pennathur Gautam
  • Rapid detection of proteins in polyacrylamide electrophoresis gels with direct red 81 and amido black
  • David Choveaux, Robert G.E. Krause, and J.P. Dean Goldring
  • Zymographic assay of oxidases using peroxidase or hemin entrapped in polyacrylamide gel
  • Native polyacrylamide gels
  • Mircea Alexandru Mateescu [and others]
  • Coomassie brilliant blue removal/disposal from gel destain and used gel stain in an environment-friendly manner
  • Biji T. Kurien and Yaser Dorri
  • Brief review of other notable protein detection methods on acrylamide gels
  • Biji T. Kurien and R. Hal Scofield
  • Ultrasensitive protein detection and imaging : comparison of lumitein, proteoSilver, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel stains
  • Guang Ren, Carl K. Okerberg, and Suresh T. Mathews
  • Common artifacts and mistakes made in electrophoresis
  • Biji T. Kurien and R. Hal Scofield
  • Claudia Arndt [and others]
  • Cationic electrophoresis
  • Engelbert Buxbaum
  • SARCOSYL-PAGE : a new electrophoretic method for the separation and immunological detection of PEGylated proteins
  • Christian Reichel
  • Tricine-SDS-PAGE
  • Syed R. Haider, Helen J. Reid, and Barry L. Sharp
  • Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis
  • Marta P. Pereira Morais [and others]
  • Protein electrophoresis in agarose gels for separating high molecular weight proteins
  • Marion L. Greaser and Chad M. Warren
  • Increase in local protein concentration by field-inversion gel electrophoresis
  • Henghang Tsai and Hon-chiu Eastwood Leung
  • Combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples
  • Kim Y.C. Fung [and others]
  • Immunofixation electrophoresis for identification of proteins and specific antibodies
  • Gyorgy Csako
  • Microchip electrophoresis, with respect to "Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease"
  • How it all began : a personal history of gel electrophoresis
  • Mohamad Reza Mohamadi [and others]
  • Foodomics approach : CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols
  • Mustafa Celebier [and others]
  • Peptide fractionation by SDS-free polyacrylamide gel electrophoresis for proteomic analysis via DF-PAGE
  • Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra
  • Tris-acetate polyacrylamide gradient gels for the simultaneous electrophoretic analysis of proteins of very high and low molecular Mass
  • Monica Cubillos-Rojas [and others]
  • SDS-PAGE for 35S immunoprecipitation and immunoprecipitation western blotting
  • Edward P. Trieu and Ira N. Targoff
  • Two-dimensional gel electrophoresis : vertical isoelectric focusing
  • Oliver Smithies
  • Yaser Dorri
  • Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids
  • Gyorgy Csako
  • Native flatbed isoelectric focusing for determining antibody clonotype distribution
  • Biji T. Kurien and R. Hal Scofield
  • Two-dimensional gel electrophoresis : glass tube-based IEF followed by SDS-PAGE
  • Hiroyuki Matsumoto [and others]
  • One-dimensional and two-dimensional immobilized metal affinity electrophoresis
  • Bao-Shiang Lee [and others]
  • Two-dimensional difference gel electrophoresis
  • Introduction to protein electrophoresis
  • Jonathan S. Minden
  • Protein identification on archived 2-D dels
  • Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori
  • Diagonal Electrophoresis for the Detection of Protein Disulfides
  • Brian McDonagh
  • Two-dimensional blue native/SDS gel electrophoresis of multiprotein complexes
  • Jean Paul Lasserre and Armelle Menard
  • Immunoelectrophoresis : a method with many faces
  • Gyorgy Csako
  • Cell surface protein biotinylation for SDS-PAGE analysis
  • Pothur R. Srinivas
  • Giuliano Elia
  • Multichannel gel electrophoresis and continuous fraction collection apparatus for high-throughput protein separation and characterization
  • Ming Dong [and others]
  • Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome
  • Xianchun Wang and Songping Liang
  • Interactions of hemoglobin in live red blood cells measured by the electrophoresis release test
  • Yan Su, Lijun Gao, and Wenbin Qin
  • Extraction of proteins from gels : a brief review
  • Biji T. Kurien and R. Hal Scofield
  • New algorithm for the simulation of SDS 2D-PAGE datasets
  • Protein quantification methods to determine protein concentration prior to electrophoresis
  • Emilio Marengo and Elisa Robotti
  • Isolation of proteins from polyacrylamide gels
  • Irene Michalk [and others]
  • Gel drying methods
  • Slava Stamova [and others]
  • Long-term, buffer-less, wet gel storage in non-sealed polyethylene bags
  • Biji T. Kurien and R. Hal Scofield
  • Brief review of other notable electrophoretic methods
  • James D. Fesmire
  • Protein stains and applications
  • J.P. Dean Goldring
  • Ranjini K. Sundaram, Natarajan Balasubramaniyan
  • Coomassie-brilliant blue staining of polyacrylamide gels
  • Claudia Arndt [and others]
  • Accelerated coomassie blue staining and destaining of SDS-PAGE gels with application of heat
  • Biji T. Kurien and R. Hal Scofield
  • Silver staining techniques of polyacrylamide gels
  • Holger Bartsch [and others]
  • Revisit of imidazole-zinc reverse stain for protein polyacrylamide gel electrophoresis
  • Han-Min Chen
  • Counterion dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic gel digestion of stained protein for mass spectrometry
  • Protein solubilization : attend to the choice of lysis buffer
  • Wei-Tao Cong [and others]
  • TEMED-enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE
  • Na Na and Jin Ouyang
  • Microwave-assisted protein staining, destaining, and in-gel/in-solution digestion of proteins
  • Jennie R. Lill and Victor J. Nesatyy
  • Electrophoretic separation and detection of metalloproteins by x-ray fluorescence mapping
  • Tripti Khare, Yasmin Chishti, and Lydia A. Finney
  • Fluorescent staining of gels
  • Engelbert Buxbaum
  • Single-step simultaneous protein staining procedure for polyacrylamide gels and nitrocellulose membranes by alta during western blot analysis
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1 online resource (xiv, 648 pages)
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