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The Resource Protein chromatography : methods and protocols, edited by Dermot Walls and Sinéad T. Loughran

Protein chromatography : methods and protocols, edited by Dermot Walls and Sinéad T. Loughran

Protein chromatography : methods and protocols
Protein chromatography
Title remainder
methods and protocols
Statement of responsibility
edited by Dermot Walls and Sinéad T. Loughran
A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins
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index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
v. 681
Protein chromatography : methods and protocols, edited by Dermot Walls and Sinéad T. Loughran
Protein chromatography : methods and protocols, edited by Dermot Walls and Sinéad T. Loughran
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
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  • Scale-up of protein purification : downstream processing issues
  • John Joseph Milne
  • Phage display : a powerful technology for the generation of high-specificity affinity reagents from alternative immune sources
  • William J.J. Finlay [and others]
  • Protein stability : enhancement and measurement
  • Ciarán Ó'Fágáin
  • Tagging recombinant proteins to enhance solubility and aid purification
  • Sinéad T. Loughran and Dermot Walls
  • Storage and lyophilization of pure proteins
  • Ciarán Ó'Fágáin and Keith Colliton
  • Synopsis of proteins and their purification
  • Differential precipitation and solubilization of proteins
  • Barry J. Ryan and Gemma K. Kinsella
  • Ion-exchange chromatography : basic principles and application
  • Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor
  • Protein quantitation and analysis of purity
  • Eva M. Campion, Sinéad T. Loughran, and Dermot Walls
  • Purification of proteins fused to maltose-binding protein
  • Mario Lebendiker and Tsafi Danieli
  • Purification of plyhistidine-tagged proteins
  • Sinéad T. Loughran, Ronan T. Bree, and Dermot Walls
  • Dermot Walls, Gary Cooney, and Sinéad T. Loughran
  • Purification of antibodies using affinity chromatography
  • Elaine Darcy [and others]
  • Optimized generation of high-affinity, high-specificity single-chain Fv Antibodies from multi-antigen immunized chickens
  • William J.J. Finlay [and others]
  • Measuring protein-protein interactions using biacore
  • Paul Leonard [and others]
  • Hydrophobic interaction chromatography
  • Brendan F. O'Connor and Philip M. Cummins
  • Fast protein liquid chromatography
  • Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan
  • Gel-filtration chromatography
  • Clinical proteomics : liquid chromatography-mass spectrometry (LC-MS) purification systems
  • Michael Henry and Paula Meleady
  • Strategies for the purification of membrane proteins
  • Sinéad M. Smith
  • Antimicrobial peptide production and purification
  • Srinivas Suda, Des Field, and Niall Barron
  • Lectin affinity chromatography (LAC)
  • Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley
  • Ciarán Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor
  • Immunoaffinity chromatography : concepts and applications
  • Jenny Fitzgerald [and others]
  • Avoiding poteolysis during protein purification
  • Barry J. Ryan and Gary T. Henehan
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2nd ed.
1 online resource (x, 426 pages)
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illustrations (some color).
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Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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