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The Resource Protein Engineering Techniques : Gateways to Synthetic Protein Universe

Protein Engineering Techniques : Gateways to Synthetic Protein Universe

Label
Protein Engineering Techniques : Gateways to Synthetic Protein Universe
Title
Protein Engineering Techniques
Title remainder
Gateways to Synthetic Protein Universe
Creator
Contributor
Subject
Language
eng
Member of
Cataloging source
MiAaPQ
Literary form
non fiction
Nature of contents
dictionaries
Series statement
SpringerBriefs in Applied Sciences and Technology Ser
Protein Engineering Techniques : Gateways to Synthetic Protein Universe
Label
Protein Engineering Techniques : Gateways to Synthetic Protein Universe
Link
http://libproxy.rpi.edu/login?url=https://ebookcentral.proquest.com/lib/rpi/detail.action?docID=4743127
Publication
Copyright
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Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Preface -- Acknowledgements -- Contents -- 1 World of Proteins: Structure-Function Relationships and Engineering Techniques -- Abstract -- 1.1 Introduction -- 1.2 Functional Characteristics of Proteins -- 1.3 Transfer of Genetic Information: From DNA to Proteins -- 1.4 Amino Acids, Building Blocks of Proteins -- 1.4.1 Non Polar Amino Acids -- 1.4.2 Charged Polar Amino Acids -- 1.4.3 Uncharged Polar Amino Acids -- 1.5 Hydrophobicity Profiles of Amino Acids -- 1.6 Acidic and Basic Properties of Amino Acids -- 1.7 Structural Organization of Proteins -- 1.7.1 Primary Structure -- 1.7.2 Secondary Structure -- 1.7.3 Tertiary Structure -- 1.7.4 Quaternary Structure -- 1.8 Motifs/Super Secondary Structures -- 1.9 Protein Stability -- 1.9.1 Ionic Interactions -- 1.9.2 Van der Waal Interactions -- 1.9.2.1 Hydrogen Bonding -- 1.9.3 Hydrophobic Interactions -- 1.10 Protein Denaturation, Renaturation and Folding Energy Landscape -- 1.11 Structure Determination of Proteins -- 1.11.1 X-Ray Crystallography -- 1.11.2 Nuclear Magnetic Resonance Spectroscopy (NMR) -- 1.12 Concept of Biomolecular and Protein Engineering -- References -- 2 Expanding the Synthetic Protein Universe by Guided Evolutionary Concepts -- Abstract -- 2.1 Directed Evolution -- 2.2 Asexual Methods -- 2.3 Random Mutagenesis -- 2.3.1 Error Prone PCR -- 2.3.2 Chemical Mutagenesis -- 2.3.3 Mutator Strains -- 2.3.4 Dual Approach to Random Chemical Mutagenesis (DuARCheM) -- 2.3.5 Rolling Circle Error-Prone PCR -- 2.3.6 Targeting Glycosylases to Embedded Arrays for Mutagenesis (TaGTEAM) -- 2.3.7 Mutagenesis by Random Insertion and Deletion -- 2.3.8 Transposon Based Random Mutagenesis -- 2.3.9 Random Mutagenesis Methods Altering the Target DNA Length -- 2.4 Focused Mutagenesis -- 2.5 Site Saturation Mutagenesis (SSM) -- 2.5.1 Cassette Mutagenesis -- 2.5.2 Sequence Saturation Mutagenesis (SeSAM)
  • 2.5.3 Single-Primer Reactions in Parallel (SPRINP) -- 2.5.4 Megaprimed and Ligase-Free -- 2.5.5 ]-PCR -- 2.5.6 PFunkel-Ominchange-OSCARR -- 2.5.7 Trimer-Dimer Mutagenesis -- 2.6 Sexual Methods -- 2.7 Homologous Recombination -- 2.8 In Vitro Homologous Recombination -- 2.8.1 DNA Shuffling -- 2.8.2 Random Priming In Vitro Recombination (RPR) -- 2.8.3 Truncated Metagenomic Gene-Specific PCR (TMGS-PCR) -- 2.8.4 Staggered Extension Process (StEP) -- 2.8.5 Random Chimeragenesis on Transient Templates (RACHITT) -- 2.8.6 Synthetic Shuffling -- 2.9 In Vivo Homologous Recombination -- 2.9.1 Cloning Performed in Yeast (CLERY) -- 2.9.2 Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) -- 2.9.3 Phage Assisted Continuous Evolution (PACE) -- 2.10 In Vitro Non-homologous Recombination Methods -- 2.10.1 Exon Shuffling -- 2.10.2 Incremental Truncation for the Creation of Hybrid Enzymes (ITCHY) -- 2.10.3 SCRATCHY -- 2.10.4 Recombined Extension on Truncated Templates (RETT) -- 2.10.5 Sequence Homology-Independent Protein Recombination (SHIPREC) -- 2.10.6 Sequence Independent Site-Directed Chimeragenesis (SISDC) -- 2.10.7 Degenerate Homo-Duplex Recombination (DHR) -- 2.10.8 Random Multi-recombinant PCR (RM-PCR) -- 2.10.9 User Friendly DNA Recombination (USERec) -- 2.10.10 Golden Gate Shuffling (GGS) Recombination -- 2.10.11 PhosphoroThioate-Based DNA Recombination Method (PTRec) -- 2.10.12 Integron -- 2.10.13 Y-Ligation Based Shuffling (YLBS) -- 2.11 Screening and Selection Techniques -- 2.12 Proteins Designed Using Directed Evolution (DE) Methods -- References -- 3 Rational Designing of Novel Proteins Through Computational Approaches -- Abstract -- 3.1 Computational Designing of Proteins -- 3.2 Rational Designing of Proteins -- 3.3 Multiple Sequence Alignment (MSA) -- 3.3.1 Few Case Studies that Employed MSA Tools for Designing Proteins
  • 3.4 Coevolutionary Analysis -- 3.5 Structure Based Designing of New Proteins -- 3.5.1 Ab Initio Methods -- 3.5.2 Fragment Based Methods -- 3.5.3 Homology Modeling -- 3.5.4 Protein Threading -- References -- 4 Generating the Fancy Protein Basket with De Novo and Combinatorial Approaches -- Abstract -- 4.1 De Novo Designing of Proteins -- 4.1.1 Building Backbone -- 4.1.2 Sequence Design and Optimization -- 4.1.3 In Silico and Experimental Validation -- 4.2 Negative Design Methods -- 4.3 Examples of De Novo Designed Proteins -- 4.4 Combinatorial/Semi-rational Approach for Protein Designing -- 4.5 Glimpse on Proteins Engineered by de Novo/Combinatorial Approaches -- References -- 5 Biotechnological and Biomedical Applications of Protein Engineering Methods -- Abstract -- 5.1 Diversified Applications of Protein Engineering Techniques -- 5.2 Industrial Applications -- 5.3 Environmental Applications -- 5.4 Biomaterial Applications -- 5.4.1 Elastin like Polypeptides (ELPs) -- 5.4.2 Silk Motifs -- 5.4.3 Coiled-Coil Motifs -- 5.4.4 Calmodulin Motifs -- 5.4.5 Ý-Sheet Forming Ionic Oligopeptides -- 5.5 Applications in Nanotechnology -- 5.6 Biosensors -- 5.7 Virus Engineering -- 5.8 Biomedical Applications -- 5.8.1 Engineered Proteins as Therapeutics -- 5.8.2 Protein Scaffolds as Therapeutics -- References
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{'f': 'http://opac.lib.rpi.edu/record=b4386568'}
Extent
1 online resource (144 pages)
Form of item
online
Isbn
9789811027321
Media category
computer
Media MARC source
rdamedia
Media type code
c
Sound
unknown sound
Specific material designation
remote

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