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The Resource Progenitor cells : methods and protocols, edited by Kimberly A. Mace, Kristin M. Braun

Progenitor cells : methods and protocols, edited by Kimberly A. Mace, Kristin M. Braun

Progenitor cells : methods and protocols
Progenitor cells
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methods and protocols
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edited by Kimberly A. Mace, Kristin M. Braun
Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types. This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Progenitor cells : methods and protocols, edited by Kimberly A. Mace, Kristin M. Braun
Progenitor cells : methods and protocols, edited by Kimberly A. Mace, Kristin M. Braun
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Includes bibliographical references and index
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online resource
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  • Isolation of adult stem cells and their differentiation to schwann cells
  • Cristina Mantovani, Giorgio Terenghi, and Susan G. Shawcross
  • Functional purification of human and mouse mammary stem cells
  • Daniela Tosoni, Pier Paolo Di Fiore, and Salvatore Pece
  • Isolation and expansion of endothelial progenitor cells derived from mouse embryonic stem cells
  • S. Bahram Bahrami, Mandana Veiseh, and Nancy J. Boudreau
  • Transcriptome analysis of Drosophila neural stem cells
  • Katrina S. Gold and Andrea H. Brand
  • Live imaging for studying asymmetric cell division in the C. elegans embryo
  • Alexia Rabilotta, Rana Amini, and Jean-Claude Labbe
  • Isolation of satellite cells from single muscle fibers from young, aged, or dystrophic muscles
  • Tol2-mediated gene transfer and in ovo electroporation of the otic placode : a powerful and versatile approach for investigating embryonic development and regeneration of the chicken inner ear
  • Stephen Freeman, Elena Chrysostomou, Koichi Kawakami, Yoshiko Takahashi, and Nicolas Daudet
  • Labeling primitive myeloid progenitor cells in Xenopus
  • Ricardo Costa, Yaoyao Chen, Roberto Paredes, and Enrique Amaya
  • Identification of oocyte progenitor cells in the zebrafish ovary
  • Bruce W. Draper
  • FACS analysis of the planarian stem cell compartment as a tool to understand regenerative mechanisms/
  • Belen Tejada Romero, Deborah J. Evans, and A. Aziz Aboobaker
  • Clonal and lineage analysis of melanocyte stem cells and their progeny in the zebrafish
  • Robert C. Tryon and Stephen L. Johnson
  • Valentina Di Foggia and Lesley Robson
  • Reconstitution of the central nervous system during salamander tail regeneration from the implanted neurospheres
  • Levan Mchedlishvili, Vladimir Mazurov, and Elly M. Tanaka
  • Following the fate of neural progenitors by homotopic/homochronic grafts in Xenopus embryos
  • Raphael Thuret and Nancy Papalopulu
  • Analyzing the angiogenic potential of Gr-1+CD11b+ immature myeloid cells from murine wounds
  • Elahe Mahdipour and Kimberly A. Mace
  • In vivo imaging of hematopoietic stem cells in the bone marrow niche
  • Oliver Barrett, Roberta Sottocornola, and Cristina Lo Celso
  • Characterizing the phenotype of murine epidermal progenitor cells : complementary whole-mount visualization and flow cytometry strategies
  • Korinna D. Henseleit, Ann P. Wheeler, Gary Warnes, and Kristin M. Braun
  • Terminal differentiation of human epidermal stem cells on micro-patterned substrates
  • Murine aggregation chimeras and wholemount imaging in airway stem cell biology
  • Ian R. Rosewell and Adam Giangreco
  • Live imaging of primitive endoderm precursors in the mouse blastocyst
  • Joanna B. Grabarek and Berenika Plusa
  • Utilizing DNA mutations to trace epithelial cell lineages in human tissues
  • Sebastian Zeki, Trevor A. Graham, and Stuart A.C. McDonald
  • NF-Ya protein delivery as a tool for hematopoietic progenitor cell expansion
  • Alevtina D. Domashenko, Susan Wiener, and Stephen G. Emerson
  • Exploring the link between human embryonic stem cell organization and fate using tension-calibrated extracellular matrix functionalized polyacrylamide gels
  • Johnathon N. Lakins, Andrew R. Chin, and Valerie M. Weaver
  • John T. Connelly
  • Isolation of circulating angiogenic cells
  • Erin E. Vaughan and Timothy O'Brien
  • Methods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine
  • Francesca Corradini, Beatrice Venturi, Graziella Pellegrini, and Michele De Luca
  • ALDH as a marker for enriching tumorigenic human colonic stem cells
  • Anitha Shenoy, Elizabeth Butterworth, and Emina H. Huang
  • Protocols for investigating microRNA functions in human neural progenitor cells
  • Sandra Almeida, Celine Delaloy, Lei Liu, and Fen-Biao Gao
  • Isolation, culture, and potentiality assessment of lung alveolar stem cells
  • Feride Oeztuerk-Winder and Juan-Jose Ventura
  • Three-dimensional in vitro culture techniques for mesenchymal stem cells
  • Fatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, and Paul G. Genever
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1 online resource (xiv, 408 pages)
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