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The Resource Plant salt tolerance : methods and protocols, edited by Sergey Shabala, Tracey Ann Cuin

Plant salt tolerance : methods and protocols, edited by Sergey Shabala, Tracey Ann Cuin

Plant salt tolerance : methods and protocols
Plant salt tolerance
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methods and protocols
Statement of responsibility
edited by Sergey Shabala, Tracey Ann Cuin
Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Plant salt tolerance : methods and protocols, edited by Sergey Shabala, Tracey Ann Cuin
Plant salt tolerance : methods and protocols, edited by Sergey Shabala, Tracey Ann Cuin
Includes index
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Includes bibliographical references and index
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online resource
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  • Single-cell sampling and analysis (SiCSA)
  • Wieland Fricke
  • Non-invasive flux measurements using microsensors : theory, limitations, and systems
  • Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, and Jian Sun
  • Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique
  • Sergey Shabala, Tracey Ann Cuin, Lana Shabala, and Ian Newman
  • Quantitative cryo-analytical scanning electron microscopy (CEDX) : an important technique useful for cell-specific localization of salt
  • Margaret McCully and Martin Canny
  • Fluorescence lifetime imaging (FLIM) measurements in salinity research
  • Olga Babourina and Zed Rengel
  • Patch-clamp protocols to study cell ionic homeostasis under saline conditions
  • Cytosolic Ca2 determinations in studying plant responses to salinity and oxidative stress
  • Anuphon Laohavisit, Renato Colaço, and Julia Davies
  • Infrared thermography in plant phenotyping for salinity tolerance
  • Richard A. James and Xavier R.R. Sirault
  • In vivo imaging of nitric oxide and reactive oxygen species using laser scanning confocal microscopy
  • Yan-Jie Xie and Wen-Biao Shen
  • Metabolomics for salinity research
  • Ute Roessner and Diane M. Beckles
  • Purification of plant plasma membranes by two-phase partitioning and measurement of H pumping
  • Anette Lund and Anja Thoe Fuglsang
  • Ana María Velarde-Buendía, René Alberto Enríquez-Figueroa, and Igor Pottosin
  • Determination of reactive oxygen species in salt-stressed plant tissues
  • Andrés Alberto Rodríguez and Edith L. Taleisnik
  • Quantification of the antioxidant activity in salt-stressed tissues
  • Jelena J. Dragišič Maksimović and Branka D. Živanovič
  • Quantification of abscisic acid, cytokinin, and auxin content in salt-stressed plant tissues
  • Petre I. Dobrev and Radomira Vankova
  • Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice
  • Aurelie Evrard, Bastiaan O.R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A.T. Johnson
  • Transformation using controlled cDNA overexpression system
  • Gábor Rigó, Csaba Papdi, and László Szabados
  • Studying plant salt tolerance with the voltage clamp technique
  • Transcriptome analysis of membrane transporters in response to salinity stress
  • Prasad Senadheera and Frans J.M. Maathuis
  • Marker-assisted selection in plant breeding for salinity tolerance / M. Ashraf, N.A. Akram, Mehboob-ur-Rahman, and M.R. Foolad
  • Transcriptomics on small samples
  • Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, and Matthew Gilliham
  • Plastid transformation for abiotic stress tolerance in plants
  • K.C. Bansal, A.K. Singh, and S.H. Wani
  • Manipulating expression of tonoplast transporters
  • Zhigang Li, Man Zhou, Qian Hu, Shane Reighard, Shuangrong Yuan, Ning Yuan, Bekir San, Dayong Li, Haiyan Jia, and Hong Luo
  • Using heterologous expression systems to characterize potassium and sodium transport activities
  • Zhong-Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, and Michael R. Blatt
  • Alonso Rodriguez, Begona Benito, and Olivier Cagnac
  • Isotope techniques to study kinetics of Na and K transport under salinity conditions
  • Dev T. Britto and Herbert J. Ronzucker
  • Trait dissection of salinity tolerance with plant phenomics
  • Bettina Berger, Bas de Regt, and Mark Tester
  • Measuring soil salinity
  • Marcus Hardie and Richard Doyle
  • Using the multifunctional xylem probe for in situ studies of plant water and ion relations under saline conditions
  • Lars H. Wegner
  • Measuring intracellular ion concentrations with multi-barrelled microelectrodes
  • Anthony J. Miller and Susan Smith
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1 online resource (xiv, 432 pages)
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