Coverart for item
The Resource Plant proteomics : methods and protocols, edited by Hervé Thiellement [and others]

Plant proteomics : methods and protocols, edited by Hervé Thiellement [and others]

Label
Plant proteomics : methods and protocols
Title
Plant proteomics
Title remainder
methods and protocols
Statement of responsibility
edited by Hervé Thiellement [and others]
Contributor
Subject
Genre
Language
eng
Summary
The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22)
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
355
Plant proteomics : methods and protocols, edited by Hervé Thiellement [and others]
Label
Plant proteomics : methods and protocols, edited by Hervé Thiellement [and others]
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1597452270
Publication
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Related Items
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Total protein extraction with TCA-acetone / Valérie Méchin, Catherine Damerval, and Michel Zivy -- Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues / Mireille Faurobert, Esther Pelpoir, and Jamila Chaïb -- Protein extraction from cereal seeds / Gérard Branlard and Emmanuelle Blancel -- Protein extraction from xylem and phloem sap / Julia Kehr and Martijn Rep -- Protein extraction from woody plants / Christophe Plomion and Céline Lalanne -- Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis / Klaas J. van Wijk, Jean-Benoit Peltier, and Lisa Giacomelli -- Isolation and subfractionation of plant mitochondria for proteomic analysis / Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar -- Extraction of nuclear proteins from root meristematic cells / Fernando González-Camacho and Francisco Javier Medina -- Isolation of nuclear proteins / Setsuko Komatsu -- Isolation of cell wall proteins from Medicago sativa stems / Bonnie S. Watson and Lloyd W. Sumner -- Plant plasma membrane protein extraction and solubilization for proteomic analysis / Véronique Santoni -- Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / Thierry Rabilloud [and others] -- Two-dimensional electrophoresis for plant proteomics / Walter Weiss and Angelika Görg -- Visible and fluorescent staining of two-dimensional gels / François Chevalier, Valérie Rofidal, and Michel Rossignol -- Two-dimensional differential in-gel electrophoresis (DIGE) of leaf and roots of Lycopersicon esculentum / Matthew Keeler [and others] -- Quantitative analysis of 2D gels / Michel Zivy -- Multivariate data analysis of proteome data / Kåre Engkilde, Susanne Jacobsen, and Ib Søndergaard -- Edman sequencing of plant proteins from 2D gels / Setsuko Komatsu -- Peptide mass fingerprinting: identification of proteins by MALDI-TOF / Nicolas Sommerer, Delphine Centeno, and Michel Rossignol -- Protein identification using nano liquid chromatography-tandem mass spectrometry / Luc Negroni -- Two-dimensional nanoflow liquid chromatography-tandem mass spectrometry of proteins extracted from rice leaves and roots / Linda Breci and Paul A. Haynes -- Separation, identification, and profiling of membrane proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS / Wojciech Szponarski [and others] -- The PROTICdb database for 2-DE proteomics / Olivier Langella, Michel Zivy, and Johann Joets -- Identification of phosphorylated proteins / Maria V. Turkina and Alexander V. Vener -- Plant proteomics and glycosylation / Anne-Catherine Fitchette [and others] -- Blue-native gel electrophoresis for the characterization of protein complexes in plants / Jesco Heinemeyer, Dagmar Lewejohann, and Hans-Peter Braun -- Electroelution of intact proteins from SDS-PAGE gels and their subsequent MALDI-TOF MS analysis / Zhentian Lei [and others] -- Generation of plant protein microarrays and investigation of antigen-antibody interactions / Birgit Kersten and Tanja Feilner -- Phosphorylation studies using plant protein microarrays / Tanja Feilner and Birgit Kersten
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781597452274&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357101'}
Extent
1 online resource (xiii, 399 pages)
Form of item
online
Isbn
9781597452274
Lccn
2006041207
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
Processing Feedback ...