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The Resource Plant protein secretion : methods and protocols, edited by Liwen Jiang

Plant protein secretion : methods and protocols, edited by Liwen Jiang

Plant protein secretion : methods and protocols
Plant protein secretion
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methods and protocols
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edited by Liwen Jiang
This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."
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  • bibliography
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Methods in molecular biology,
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Plant protein secretion : methods and protocols, edited by Liwen Jiang
Plant protein secretion : methods and protocols, edited by Liwen Jiang
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Includes bibliographical references and index
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  • Yonglun Zeng
  • Using microscopy tools to visualize autophagosomal structures in plant cells
  • Weili Lin,
  • Xiaohong Zhuang
  • 24.
  • Analysis of plant autophagy
  • Liang Chen,
  • Faqiang Li,
  • Shi Xiao
  • 3.
  • Bioinformatics analysis of protein secretion in plants
  • Liyuan Chen
  • 4.
  • Proteomic analysis of secreted proteins from cell microenvironment
  • Subash Adhikari,
  • Lan Chen,
  • Peiwu Huang,
  • Ruijun Tian
  • 1.
  • 5.
  • Using homology modeling to understand the structural basis of specific interaction of a plant-specific AtSar1a-AtSec23a pair involved in protein ER export
  • Yap-Shing Nim,
  • Shuangli Sun,
  • Kam-Bo Wong
  • 6.
  • Analysis of Golgi-mediated protein traffic in plant cells
  • Wenjin Shen,
  • Zhidan Xiao,
  • Jinbo Shen,
  • An overview of protein secretion in yeast and animal cells
  • Caiji Gao
  • 7.
  • Analysis of membrane protein topology in the plant secretory pathway
  • Jinya Guo,
  • Yansong Miao,
  • Yi Cai
  • 8.
  • Semiautomatic segmentation of plant Golgi stacks in electron tomograms using 3dmod
  • Keith Ka Ki Mai,
  • Byung-Ho Kang
  • Yusong Guo,
  • 9.
  • 3D printing of plant Golgi stacks from their electron tomographic models
  • Keith Ka Ki Mai,
  • Madison J. Kang,
  • Byung-Ho Kang
  • 10.
  • Transient expression of chimeric fluorescent reporter proteins in pollen tubes to study protein polar secretion and dynamics
  • Guitao Zhong,
  • Ronghe Liu,
  • Menglong Zhuang,
  • Feng Yang,
  • Hao Wang
  • 11.
  • Analysis of actin-based intracellular trafficking in pollen tubes
  • Yuxiang Jiang,
  • Meng Zhang,
  • Shanjin Huang
  • 12.
  • Analysis of phragmoplast kinetics during plant cytokinesis
  • Pantelis Livanos,
  • Mayank Chugh,
  • Xiao Tang
  • Sabine Müller
  • 13.
  • Immunofluorescence analysis of membrane-associated proteins for calthrin-mediated endocytosis in plant root cells
  • Chao Wang,
  • Xu Yan,
  • Tingting Meng,
  • Tianwei Hu,
  • Jianwei Pan
  • 14.
  • In vivo interaction studies by measuring Förster resonance energy transfer through fluorescence lifetime imaging microscopy (FRET/FLIM)
  • 2.
  • Florian Fässler,
  • Peter Pimpl
  • 15.
  • Analysis of nano-body-epitope interactions in living cells via quantitative protein transport assays
  • Simone Früholz and Peter Pimpl
  • 16.
  • A secretion system for cargo protein identification of vacuolar sorting receptors
  • Jinbo Shen
  • 17.
  • Identifying novel regulators of vacuolar trafficking by combining fluorescence imaging-based forward genetic screening and in vitro pollen germination
  • An overview of protein secretion in plant cells
  • Qiang-Nan Feng,
  • Yan Zhang
  • 18.
  • Measuring plan protein secretion
  • Emily R. Larson -- 19.
  • Transient secretory enzyme expression in leaf protoplasts to characterize SNARE functional classes in conventional and unconventional secretion
  • Di Sansebastiano Gian Pietro,
  • Barozzi Fabrizio
  • 20.
  • The organelle pH estimate and measurement in plant secratory pathway
  • Kin Pau Chung,
  • Jinbo Shen
  • 21.
  • Analysis of exocyst-positive organelle (EXPO)-mediated unconventional protein secretion (UPS) in plant cells
  • Yu Ding,
  • Juan Wang
  • 22.
  • Isolation of the plant exocyst complex
  • King Pong Leung,
  • Wilson Chun Yu Lau
  • 23.
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