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The Resource Plant immunity : methods and protocols, edited by John M. McDowell

Plant immunity : methods and protocols, edited by John M. McDowell

Plant immunity : methods and protocols
Plant immunity
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methods and protocols
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edited by John M. McDowell
A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Plant immunity : methods and protocols, edited by John M. McDowell
Plant immunity : methods and protocols, edited by John M. McDowell
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Includes bibliographical references and index
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online resource
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Studying NB-LRR immune receptor localization by agroinfiltration transient expression / P. Cournoyer and S.P. Dinesh-Kumar -- Fragment complementation and co-immunoprecipitation assays for understanding R protein structure and function / P. Moffett -- Purification of resistance protein complexes using a biotinylated affinity (HPB) tag / Y. Qi and F. Katagiri -- Biochemical purification of native immune protein complexes / J.M. Elmore and G. Coaker -- Chromatin immunoprecipitation to identify global targets of WRKY transcription factor family members involved in plant immunity / M. Roccaro and I.E. Somssich -- Dose-response to and systemic movement of dexamethasone in the GVG-inducible transgene system in arabidopsis / X. Geng and D. Mackey -- Quantifying alternatively spliced mRNA via capillary electrophoresis / X.C. Zhang and W. Gassmann -- Constructing haustorium-specific cDNA libraries from rust fungi / A.M. Catanzariti [and others] -- Microaspiration of esophageal gland cells and cDNA library construction for identifying parasitism genes of plant-parasitic nematodes / R.S. Hussey, G. Huang and R. Allen -- Construction of pseudomonas syringae pv. Tomato DC3000 mutant and polymutant strains / B.H. Kvitko and A. Collmer -- A straightforward protocol for electro-transformation of phytophthora capsici zoospores / E. Huitema, M. Smoker and S. Kamoun ---Propagation, storage, and assays with hyaloperonospora arabidopsidis: A model oomycete pathogen of arabidopsis / J.M. McDowell [and others] -- Assaying effector function in planta using double-barreled particle bombardment / S.D. Kale and B.M. Tyler -- Assays for effector-mediated suppression of programmed cell death in yeast / Y. Wang and Q. Huang -- Purification of effector-target protein complexes via transient expression in nicotiana benthamiana / J. Win, S. Kamoun and A.M. Jones --Imaging fluorescently tagged phytophthora effector proteins inside infected plant tissue / P.C. Boevink, P.R. Birch and S.C. Whisson -- Immunolocalization of pathogen effectors / E. Kemen, K. Mendgen and R.T. Voegele -- Laser capture microdissection of nematode feeding cells / N. Ithal and M.G. Mitchum -- Laser microdissection of plant-fungus interaction sites and isolation of RNA for downstream expression profiling / D. Chandran, N. Inada and M.C. Wildermuth -- Global expression profiling of RNA from laser microdissected cells at fungal-plant interaction sites / D. Chandran, G. Hather and M.C. Wildermuth-Visualizing cellular dynamics in plant-microbe interactions using fluorescent-tagged proteins / W. Underwood, S. Koh and S.C. Somerville
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1 online resource (xv, 295 pages).
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