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The Resource Photosynthesis research protocols, edited by Robert Carpentier

Photosynthesis research protocols, edited by Robert Carpentier

Label
Photosynthesis research protocols
Title
Photosynthesis research protocols
Statement of responsibility
edited by Robert Carpentier
Contributor
Subject
Genre
Language
eng
Summary
The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
v. 684
Photosynthesis research protocols, edited by Robert Carpentier
Label
Photosynthesis research protocols, edited by Robert Carpentier
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-60761-925-3
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants -- Isolation of photosystem I submembrane fractions -- Isolation of photosystem II reaction center complexes from plants -- Methods for the isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 6803 -- Purification and crystallization of oxygen-evolving photosystem II core complex from thermophilic cyanobacteria -- Isolation of cytochrome b6f complex from grana and stroma membranes from spinach chloroplasts -- Purification and crystallization of the cyanobacterial cytochrome B6F complex -- Purification of plastocyanin and cytochrome C6 from plants, green algae, and cyanobacteria -- Isolation and identification of chloroplast lipids -- Isolation and purification of CP43 and CP47 photosystem II proximal antenna complexes from plants -- Preparation of native and recombinant light-harvesting chlorophyll-a/b complex -- Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions -- Protein targeting across and into chloroplast membranes -- Proteomic analysis of thylakoid membranes -- Thylakoid phosphoproteins: Identification of phosphorylation sites -- Direct detection of free radicals and reactive oxygen species in thylakoids -- Assay of photoinhibition and heat inhibition of photosystem II in higher plants -- Photosystem II reconstitution into proteoliposomes and methodologies for structure-function characterization -- Physical and chemical immobilization methods of photosynthetic materials -- Identifying chloroplast biogenesis and signalling mutants in arabidopsis thaliana -- Expression of genes in cyanobacteria: Adaptation of endogenous plasmids as platforms for high-level gene expression in synechococcus sp. PCC 7002 -- Construction of gene interruptions and gene deletions in the cyanobacterium synechocystis sp. Strain PCC 6803 -- A simple method for chloroplast transformation in Chlamydomonas reinhardtii -- Rapid isolation of intact chloroplasts from spinach leaves -- Mechanical isolation of bundle sheath cell strands and thylakoids from leaves of C4 grasses -- Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves -- Quantifying the amount and activity of Rubisco in leaves -- Purification of Rubisco activase from leaves or after expression in escherichia coli -- Rubisco activase activity assays -- Quantification of Rubisco activase content in leaf extracts
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781607619253&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357567'}
Edition
2nd ed.
Extent
1 online resource (xiv, 395 pages)
Form of item
online
Isbn
9781607619253
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote

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