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The Resource Pediatric hematology : methods and protocols, edited by Nicholas J. Goulden, Colin G. Steward

Pediatric hematology : methods and protocols, edited by Nicholas J. Goulden, Colin G. Steward

Label
Pediatric hematology : methods and protocols
Title
Pediatric hematology
Title remainder
methods and protocols
Statement of responsibility
edited by Nicholas J. Goulden, Colin G. Steward
Contributor
Subject
Language
eng
Summary
Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular medicine
Series volume
91
Pediatric hematology : methods and protocols, edited by Nicholas J. Goulden, Colin G. Steward
Label
Pediatric hematology : methods and protocols, edited by Nicholas J. Goulden, Colin G. Steward
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592594336
Publication
Related Contributor
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Molecular diagnosis of Fanconi anemia and dyskeratosis congenita / A.J. Tipping [and others] -- Molecular diagnosis of Diamond-Blackfan anemia / S. Ball and K. Orfali -- Antenatal diagnosis of hemoglobinopathies / J.M. Old -- Prenatal diagnosis of hemophilia / D. Stirling -- Human platelet antigen genotyping by PCR-SSP in neonatal/fetal alloimmune thrombocytopenia / C. Hurd and G. Lucas -- Multiplex PCR for the detection of the factor V Leiden and prothrombin 20210A mutations / A.M. Sproul and E.A. Chalmers -- Molecular diagnosis of congenital immunodeficiency / D. Eastwood, K.C. Gilmour and H.B. Gaspar -- Molecular techniques to improve outcome in childhood ALL / F.W. van Delft and V. Saha -- Molecular cytogenetics in childhood leukemia / C.J. Harrison [and others] -- Fluorescent IgH fingerprinting to assess minimal residual disease in childhood B-lineage ALL / P.A. Evans and R.G. Owen -- Real-time quantitative RT-PCR to detect fusion gene transcripts associated with AML / R. Flora and D. Grimwade -- Real-time PCR to detect minimal residual disease in childhood ALL / C. Eckert and O. Landt -- Oligonucleotide microarray analysis of gene expression in leukemia / F.W. van Delft and L.K. Jones -- HLA low-resolution genotyping for hematopoietic stem cell transplantation / J. Harvey, J. Price and A. Stanton -- DNA sequencing as a tissue-typing tool / P.P. Dunn [and others] -- Analysis of chimerism after stem cell transplantation / P. Bader and H. Kreyenberg
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592594337&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356750'}
Extent
1 online resource (xii, 272 pages)
Form of item
online
Isbn
9781592594337
Lccn
2003004723
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

Library Locations

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