The Resource PCR primer design, edited by Anton Yuryev
PCR primer design, edited by Anton Yuryev
- Summary
- In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research
- Language
- eng
- Extent
- 1 online resource (xiii, 431 pages)
- Note
- Title from title screen (viewed Nov. 20, 2007)
- Contents
-
- Physical principles and visual-OMP software for optimal PCR design / J. SantaLucia, Jr.
- OLIGO 7 primer analysis software / W. Rychlik
- Selection for 3'-end triplets for polymerase chain reaction primers / K. Onodera
- The reference point method in primer design / T. Kampke
- PCR primer design using statistical modeling / A. Yuryev
- Developing a statistical model for primer design / J. Huang and A. Yuryev
- Gst-prime: An algorithm for genome-wide primer design / D. Leister and C. Varotto
- Genome-scale probe and primer design with primegens / G.P. Srivastava and D. Xu
- SNPbox: Web-based high-throughput primer design with an eye for repetitive sequences / S. Weckx [and others]
- Fast masking of repeated primer binding sites in eukaryotic genomes / R. Andreson, L. Kaplinski and M. Remm
- Degenerate primer design: Theoretical analysis and the hyden program / C. Linhart and R. Shamir
- An iterative method for selecting degenerate multiplex PCR primers / R. Souvenir [and others]
- Primer design for multiplexed genotyping / L. Kaderali
- Multiplx: Automatic grouping and evaluation of PCR primers / L. Kaplinski and M. Remm
- Multiprimer: A system for microarray PCR primer design / R. Fernandes and S. Skiena
- Modified oligonucleotides as tools for allele-specific amplification / M. Strerath [and others]
- AlleleID: A pathogen detection and identification system / A. Apte and S. Singh
- Designing primers for whole genome PCR scanning using the software package GenoFrag: A software package for the design of primers dedicated to whole-genome scanning by LR-PCR / N. Ben Zakour and Y. Le Loir
- Designing PCR primer for DNA methylation mapping / L.C. Li
- Bisearch: EPCR tool for native or bisulfite-treated genomic template / T. Aranyi and G.E. Tusnady
- Graphical design of primers with perlprimer / O. Marshall
- GST-PRIME: an algorithm for genome-wide primer design / D. Leister and C. Varotto
- Genome-scale probe and primer design with PRIMEGENS / G.P. Srivastava and D. Xu
- Isbn
- 9781588297259
- Label
- PCR primer design
- Title
- PCR primer design
- Statement of responsibility
- edited by Anton Yuryev
- Subject
-
- Polynucleotides
- Chemicals and Drugs
- Molecular Probes
- Sequence Analysis, DNA
- Models, Statistical
- Specialty Uses of Chemicals
- Sequence Analysis
- Oligodeoxyribonucleotides
- Health Care Quality, Access, and Evaluation
- Nucleic Acids, Nucleotides, and Nucleosides
- Epidemiologic Methods
- Delivery of Health Care
- Statistics as Topic
- Genetic Techniques
- Health Care Evaluation Mechanisms
- DNA Primers
- Quality of Health Care
- Analytical, Diagnostic and Therapeutic Techniques and Equipment
- Nucleic Acid Amplification Techniques
- Investigative Techniques
- DNA Probes
- Chemical Actions and Uses
- Models, Theoretical
- DNA polymerases
- Nucleic Acid Probes
- Laboratory Chemicals
- Nucleotides
- Oligonucleotides
- Polymerase chain reaction
- Public Health
- Environment and Public Health
- Polymerase Chain Reaction
- Language
- eng
- Summary
- In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research
- Cataloging source
- YUM
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- Series statement
- Methods in molecular biology
- Series volume
- 402
- Label
- PCR primer design, edited by Anton Yuryev
- Note
- Title from title screen (viewed Nov. 20, 2007)
- Related Authorities
-
- Health Care Quality, Access, and Evaluation
- DNA Primers
- Polymerase chain reaction
- Chemicals and Drugs
- Health Care Evaluation Mechanisms
- Oligonucleotides
- Quality of Health Care
- Polynucleotides
- Genetic Techniques
- Sequence Analysis
- Models, Statistical
- Delivery of Health Care
- Chemical Actions and Uses
- DNA Probes
- Analytical, Diagnostic and Therapeutic Techniques and Equipment
- Nucleotides
- Statistics as Topic
- Nucleic Acid Probes
- Models, Theoretical
- Nucleic Acid Amplification Techniques
- Laboratory Chemicals
- Nucleic Acids, Nucleotides, and Nucleosides
- Oligodeoxyribonucleotides
- Specialty Uses of Chemicals
- Epidemiologic Methods
- Molecular Probes
- Environment and Public Health
- Polymerase Chain Reaction
- Investigative Techniques
- DNA polymerases
- Sequence Analysis, DNA
- Public Health
- Related Subjects
-
- Polynucleotides
- Chemicals and Drugs
- Molecular Probes
- Sequence Analysis, DNA
- Models, Statistical
- Specialty Uses of Chemicals
- Sequence Analysis
- Oligodeoxyribonucleotides
- Health Care Quality, Access, and Evaluation
- Nucleic Acids, Nucleotides, and Nucleosides
- Epidemiologic Methods
- Delivery of Health Care
- Statistics as Topic
- Genetic Techniques
- Health Care Evaluation Mechanisms
- DNA Primers
- Quality of Health Care
- Analytical, Diagnostic and Therapeutic Techniques and Equipment
- Nucleic Acid Amplification Techniques
- Investigative Techniques
- DNA Probes
- Chemical Actions and Uses
- Models, Theoretical
- DNA polymerases
- Nucleic Acid Probes
- Laboratory Chemicals
- Nucleotides
- Oligonucleotides
- Polymerase chain reaction
- Public Health
- Environment and Public Health
- Polymerase Chain Reaction
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
- txt
- Content type MARC source
- rdacontent
- Contents
- Physical principles and visual-OMP software for optimal PCR design / J. SantaLucia, Jr. -- OLIGO 7 primer analysis software / W. Rychlik -- Selection for 3'-end triplets for polymerase chain reaction primers / K. Onodera -- The reference point method in primer design / T. Kampke -- PCR primer design using statistical modeling / A. Yuryev -- Developing a statistical model for primer design / J. Huang and A. Yuryev -- Gst-prime: An algorithm for genome-wide primer design / D. Leister and C. Varotto -- Genome-scale probe and primer design with primegens / G.P. Srivastava and D. Xu -- SNPbox: Web-based high-throughput primer design with an eye for repetitive sequences / S. Weckx [and others] -- Fast masking of repeated primer binding sites in eukaryotic genomes / R. Andreson, L. Kaplinski and M. Remm -- Degenerate primer design: Theoretical analysis and the hyden program / C. Linhart and R. Shamir -- An iterative method for selecting degenerate multiplex PCR primers / R. Souvenir [and others] -- Primer design for multiplexed genotyping / L. Kaderali -- Multiplx: Automatic grouping and evaluation of PCR primers / L. Kaplinski and M. Remm -- Multiprimer: A system for microarray PCR primer design / R. Fernandes and S. Skiena -- Modified oligonucleotides as tools for allele-specific amplification / M. Strerath [and others] -- AlleleID: A pathogen detection and identification system / A. Apte and S. Singh -- Designing primers for whole genome PCR scanning using the software package GenoFrag: A software package for the design of primers dedicated to whole-genome scanning by LR-PCR / N. Ben Zakour and Y. Le Loir -- Designing PCR primer for DNA methylation mapping / L.C. Li -- Bisearch: EPCR tool for native or bisulfite-treated genomic template / T. Aranyi and G.E. Tusnady -- Graphical design of primers with perlprimer / O. Marshall -- GST-PRIME: an algorithm for genome-wide primer design / D. Leister and C. Varotto -- Genome-scale probe and primer design with PRIMEGENS / G.P. Srivastava and D. Xu
- http://library.link/vocab/cover_art
- https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781588297259&userID=ebsco-test&password=ebsco-test
- Dimensions
- unknown
- http://library.link/vocab/discovery_link
- {'f': 'http://opac.lib.rpi.edu/record=b3357088'}
- Extent
- 1 online resource (xiii, 431 pages)
- File format
- unknown
- Form of item
- online
- Isbn
- 9781588297259
- Lccn
- 2007925517
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
- c
- Other physical details
- illustrations.
- Quality assurance targets
- unknown
- Reformatting quality
- unknown
- Sound
- unknown sound
- Specific material designation
- remote
Subject
- Analytical, Diagnostic and Therapeutic Techniques and Equipment
- Chemical Actions and Uses
- Chemicals and Drugs
- DNA Primers
- DNA Probes
- DNA polymerases
- Delivery of Health Care
- Environment and Public Health
- Epidemiologic Methods
- Genetic Techniques
- Health Care Evaluation Mechanisms
- Health Care Quality, Access, and Evaluation
- Investigative Techniques
- Laboratory Chemicals
- Models, Statistical
- Models, Theoretical
- Molecular Probes
- Nucleic Acid Amplification Techniques
- Nucleic Acid Probes
- Nucleic Acids, Nucleotides, and Nucleosides
- Nucleotides
- Oligodeoxyribonucleotides
- Oligonucleotides
- Polymerase Chain Reaction
- Polymerase chain reaction
- Polynucleotides
- Public Health
- Quality of Health Care
- Sequence Analysis
- Sequence Analysis, DNA
- Specialty Uses of Chemicals
- Statistics as Topic
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.lib.rpi.edu/portal/PCR-primer-design-edited-by-Anton/Dc6GtVMczmU/" typeof="WorkExample http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.lib.rpi.edu/portal/PCR-primer-design-edited-by-Anton/Dc6GtVMczmU/">PCR primer design, edited by Anton Yuryev</a></span> - <span property="offers" typeOf="Offer"><span property="offeredBy" typeof="Library ll:Library" resource="http://link.lib.rpi.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.lib.rpi.edu/">Rensselaer Libraries</a></span></span></span></span></div>
