Coverart for item
The Resource Neural stem cells : methods and protocols, edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos

Neural stem cells : methods and protocols, edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos

Label
Neural stem cells : methods and protocols
Title
Neural stem cells
Title remainder
methods and protocols
Statement of responsibility
edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos
Contributor
Subject
Genre
Language
eng
Summary
Annotation
Member of
Cataloging source
N$T
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
198
Summary expansion
In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics
Neural stem cells : methods and protocols, edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos
Label
Neural stem cells : methods and protocols, edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592591868
Publication
Related Contributor
Related Location
Related Agents
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Part I. Isolation and Culture of NSCs -- 1. Neural Differentiation of Embryonic Stem Cells / K. Sue O'Shea -- 2. Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens / Eric D. Laywell [and others] -- 3. Isolation of Stem and Precursor Cells from Fetal Tissue / Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao -- 4. Olfactory Ensheathing Cells : Isolation and Culture from the Rat Olfactory Bulb / Susan C. Barnett and A. Jane Roskams -- 5. Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium / Edmund Au and A. Jane Roskams -- 6. Production of Immortalized Human Neural Crest Stem Cells / Seung U. Kim [and others] -- 7. Adult Rodent Spinal Cord Derived Neural Stem Cells : Isolation and Characterization / Lamya S. Shihabuddin -- 8. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood / Shijie Song and J. Sanchez-Ramos -- 9. Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers / Erin Lavik [and others] -- Part II. Characterization of NSCs In Vitro -- A. Cellular Techniques -- 10. Analysis of Cell Generation in the Telencephalic Neuroepithelium / Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide -- 11. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures / Angelo L. Vescovi, Rossella Galli, and Angela Gritti -- 12. Assessing the Involvement of Telomerase in Stem Cell Biology / Mark P. Mattson, Peisu Zhang, and Weiming Fu -- 13. Detection of Telomerase Activity in Neural Cells / Karen R. Prowse -- 14. In Vitro Assays for Neural Stem Cell Differentiation / Marcel M. Daadi -- 15. Electron Microscopy and Lac-Z Labeling / Bela Kosaras and Evan Snyder -- B. Electrophysiological Techniques -- 16. Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells / David S.K. Magnuson and Dante J. Morassutti -- C. Molecular Techniques -- 17. Fluorescence In Situ Hybridization / Barbara A. Tate and Rachel L. Ostroff -- 18. RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells / Theresa E. Gratsch -- 19. Differential Display : Isolation of Novel Genes / Theresa E. Gratsch -- 20. Cell Labeling and Gene Misexpression by Electroporation / Terence J. Van Raay and Michael R. Stark -- 21. Gene Therapy Using Neural Stem Cells / Luciano Conti and Elena Cattaneo -- 22. Modeling Brain Pathologies Using Neural Stem Cells / Simonetta Siplone and Elena Cattaneo -- Part III. Utilization/Characterization of NSCs In Vivo -- A. Endogenous Pools of Stem/Progenitor Cells -- 23. Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model / Marcel M. Daadi -- 24. Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods / Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano -- 25. Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Sanjay S.P. Magavi and Jeffrey D. Macklis283 -- 26. Immunocytochemical Analysis of Neuronal Differentiation / Sanjay S.P. Magavi and Jeffrey D. Macklis -- 27. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S.P. Magavi, and Jeffrey D. Macklis -- B. Transplantation -- 28. Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- 29. Optimizing Stem Cell Grafting into the CNS / Scott R. Whittemore [and others] -- 30. Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos / Lorenzo Magrassi -- 31. Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells / Tanja Zigova and Mary B. Newman -- 32. Routes of Stem Cell Administration in the Adult Rodent / Alison E. Willing [and others]
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9780896039643&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356720'}
Extent
1 online resource (xiv, 381 pages)
Form of item
online
Isbn
9780896039643
Lccn
2001051471
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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