The Resource Monoclonal antibody protocols, edited by William C. Davis
Monoclonal antibody protocols, edited by William C. Davis
- Summary
- Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory
- Language
- eng
- Extent
- 1 online resource (x, 264 pages)
- Note
- Source of title; title from title screen
- Contents
-
- Methods of immunization to enhance the immune response to specific antigens in vivo in preparation for fusions yielding monoclonal antibodies / Jon A. Rudbach, John L. Cantrell, J.T. Ulrich
- Methods of immunization to enhance the immune response to specific antigens in vitro / Margaret E. Schelling
- Culture conditions that optimize outgrowth of hybridomas / Mary J. Hamilton, William C. Davis
- Production of stable heterohybridomas producing human monoclonal antibodies / Morris D. Cooper, Risa Kirkpatrick
- Production of stable bovine-murine interspecies hybrids / Brenda V. Jones, Christopher J. Howard
- Production of monoclonal antibodies in swine / Araceli L. Lumanglas, Bosco Shang Wang
- Production of monoclonal antibodies in horses / Lance E. Perryman, Patricia H. Mason
- Use of electric-field-mediated cell fusion to produce hybridomas secreting monoclonal antibodies / Xi Zhao
- Rapid isotyping of mouse monoclonal antibodies / Philippe Pouletty
- Methods of measuring antibodies in sera/plasma, ascites, and tissue-culture media / Jon A. Rudbach, John L. Cantrell, J.T. Ulrich
- Measurement of immunoglobulin synthesis using the ELISPOT assay / Morris D. Cooper, Risa Kirkpatrick
- Use of monoclonal antibodies in immunohistochemistry / Shan-Rong Shi, Jeffrey B. Prince, Christopher M. Jones, Krishan L. Kalra, Atul K. Tandon
- Use of monoclonal antibodies in immunoelectron microscopy / Carlos E. Suarez, Ruth Brown
- Use of monoclonal antibodies for Western blotting with enhanced chemiluminescent detection / Susan J. Fowler
- The use of directly and indirectly labeled monoclonal antibodies in flow cytometry / Carleton C. Stewart, Sigrid J. Stewart
- Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules / William C. Davis, Jennifer E. Davis, Mary J. Hamilton
- In vivo and in vitro production of monoclonal antibodies : bioreactors vs immune ascites / Uwe Marx, Wolfram Merz
- Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose / Thorkild C. Bøg-Hansen
- Detection, purification, and utilization of murine monoclonal IgM antibodies / Ian D. Walker
- Preparation and use of immunoaffinity columns with monoclonal antibodies without purification from ascites and tissue-culture medium / Thorkild C. Bøg-Hansen, William C. Davis
- Antigen purification by monoclonal antibody immunoaffinity chromatography / Alberta L. Brassfield
- Coupling of monoclonal antibodies with fluorophores / Rosaria P. Haugland
- Coupling of monoclonal antibodies with biotin / Rosaria P. Haugland, Wendy W. You
- Coupling of monoclonal antibodies with enzymes / Rosaria P. Haugland
- Use of monoclonal antibodies with magnetic particles to separate cell subpopulations by negative selection / Dennis E. Vaccaro, Joan E. Markinac
- Use of monoclonal antibodies with magnetic particles to separate cell subpopulations by positive selection / Dennis E. Vaccaro, Joan E. Markinac
- Isbn
- 9781592595327
- Label
- Monoclonal antibody protocols
- Title
- Monoclonal antibody protocols
- Statement of responsibility
- edited by William C. Davis
- Language
- eng
- Summary
- Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory
- Cataloging source
- YUS
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- Series statement
- Methods in molecular biology
- Series volume
- v. 45
- Label
- Monoclonal antibody protocols, edited by William C. Davis
- Note
- Source of title; title from title screen
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
- txt
- Content type MARC source
- rdacontent
- Contents
- Methods of immunization to enhance the immune response to specific antigens in vivo in preparation for fusions yielding monoclonal antibodies / Jon A. Rudbach, John L. Cantrell, J.T. Ulrich -- Methods of immunization to enhance the immune response to specific antigens in vitro / Margaret E. Schelling -- Culture conditions that optimize outgrowth of hybridomas / Mary J. Hamilton, William C. Davis -- Production of stable heterohybridomas producing human monoclonal antibodies / Morris D. Cooper, Risa Kirkpatrick -- Production of stable bovine-murine interspecies hybrids / Brenda V. Jones, Christopher J. Howard -- Production of monoclonal antibodies in swine / Araceli L. Lumanglas, Bosco Shang Wang -- Production of monoclonal antibodies in horses / Lance E. Perryman, Patricia H. Mason -- Use of electric-field-mediated cell fusion to produce hybridomas secreting monoclonal antibodies / Xi Zhao -- Rapid isotyping of mouse monoclonal antibodies / Philippe Pouletty -- Methods of measuring antibodies in sera/plasma, ascites, and tissue-culture media / Jon A. Rudbach, John L. Cantrell, J.T. Ulrich -- Measurement of immunoglobulin synthesis using the ELISPOT assay / Morris D. Cooper, Risa Kirkpatrick -- Use of monoclonal antibodies in immunohistochemistry / Shan-Rong Shi, Jeffrey B. Prince, Christopher M. Jones, Krishan L. Kalra, Atul K. Tandon -- Use of monoclonal antibodies in immunoelectron microscopy / Carlos E. Suarez, Ruth Brown -- Use of monoclonal antibodies for Western blotting with enhanced chemiluminescent detection / Susan J. Fowler -- The use of directly and indirectly labeled monoclonal antibodies in flow cytometry / Carleton C. Stewart, Sigrid J. Stewart -- Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules / William C. Davis, Jennifer E. Davis, Mary J. Hamilton -- In vivo and in vitro production of monoclonal antibodies : bioreactors vs immune ascites / Uwe Marx, Wolfram Merz -- Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose / Thorkild C. Bøg-Hansen -- Detection, purification, and utilization of murine monoclonal IgM antibodies / Ian D. Walker -- Preparation and use of immunoaffinity columns with monoclonal antibodies without purification from ascites and tissue-culture medium / Thorkild C. Bøg-Hansen, William C. Davis -- Antigen purification by monoclonal antibody immunoaffinity chromatography / Alberta L. Brassfield -- Coupling of monoclonal antibodies with fluorophores / Rosaria P. Haugland -- Coupling of monoclonal antibodies with biotin / Rosaria P. Haugland, Wendy W. You -- Coupling of monoclonal antibodies with enzymes / Rosaria P. Haugland -- Use of monoclonal antibodies with magnetic particles to separate cell subpopulations by negative selection / Dennis E. Vaccaro, Joan E. Markinac -- Use of monoclonal antibodies with magnetic particles to separate cell subpopulations by positive selection / Dennis E. Vaccaro, Joan E. Markinac
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- Dimensions
- unknown
- http://library.link/vocab/discovery_link
- {'f': 'http://opac.lib.rpi.edu/record=b3356790'}
- Extent
- 1 online resource (x, 264 pages)
- File format
- unknown
- Form of item
- online
- Isbn
- 9781592595327
- Lccn
- 95013504
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
- c
- Other physical details
- illustrations (some color).
- Quality assurance targets
- unknown
- Reformatting quality
- unknown
- Reproduction note
- Electronic reproduction.
- Sound
- unknown sound
- Specific material designation
- remote
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
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