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The Resource Matrix metalloproteinase protocols, edited by Ian M. Clark

Matrix metalloproteinase protocols, edited by Ian M. Clark

Label
Matrix metalloproteinase protocols
Title
Matrix metalloproteinase protocols
Statement of responsibility
edited by Ian M. Clark
Contributor
Subject
Genre
Language
eng
Summary
From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
151
Matrix metalloproteinase protocols, edited by Ian M. Clark
Label
Matrix metalloproteinase protocols, edited by Ian M. Clark
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592590462
Publication
Related Contributor
Related Location
Related Agents
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Part 1 -- MMPs and TIMPs -- an overview of the field
  • Part II -- Expression and purification of MMPs and TIMPs
  • Detection of MMPs and TIMPs
  • Assay of MMP and TIMP activities
  • Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens [and others] -- Expression of mmps and timps in mammalian cells / K.M. Yeow [and others] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong [and others] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang [and others] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9780896037335&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356937'}
Extent
1 online resource (xv, 545 pages)
Form of item
online
Isbn
9780896037335
Lccn
99087618
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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