Coverart for item
The Resource Immunocytochemical methods and protocols, edited by Lorette C. Javois

Immunocytochemical methods and protocols, edited by Lorette C. Javois

Label
Immunocytochemical methods and protocols
Title
Immunocytochemical methods and protocols
Statement of responsibility
edited by Lorette C. Javois
Contributor
Subject
Language
eng
Member of
Cataloging source
YNG
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 34
Immunocytochemical methods and protocols, edited by Lorette C. Javois
Label
Immunocytochemical methods and protocols, edited by Lorette C. Javois
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/089603285X
Publication
Related Contributor
Related Location
Related Agents
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Antibody preparation -- Overview of antibody use in immunocytochemistry -- Purification of antibodies using ammonium sulfate fractionation or gel filtration -- Purification of antibodies using ion-exchange chromatography -- Purification of antibodies using affinity chromatography -- Purification of antibodies using protein A-sepharose and FPLC -- Conjugation of fluorochromes to antibodies -- Biotinylation of antibodies -- Tissue preparation for light microscopic analyses -- Overview of cell fixation and permeabilization -- Preparation of frozen sections for analysis -- Processing of cell-touch preparations -- Processing of cytological specimens -- Processing of tissue-culture cells -- Processing of tissue specimens -- Light microscopic detection systems -- Overview of fluorophores -- Direct immunofluorescent labeling of cells -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells -- Fluorescent labeling of surface or intracellular antigens in whole-amounts -- Overview of antigen detection through enzymatic activity -- Peroxidase-antiperoxidase (PAP) method -- Avidin-biotin complex (ABC) method -- Labeled avidin binding (LAB) method -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue -- ELISA on attachment-dependent or suspension grown cells -- Use of immunogold with silver enhancement -- Fluorescence-activated cell sorter (FACS) analyses -- Overview of flow cytometry and fluorescent probes for cytometry -- Tissue disaggregation -- Indirect immunofluorescent labeling of viable cells -- Indirect immunofluorescent labeling of fixed cells -- Fluorescent labeling of DNA -- Deparaffinization and processing of pathologic material -- Assay for phagocytosis -- Assay for filamentous actin -- Assay for chemoattractant binding -- Assay for oxidative metabolism -- Colloidal gold detection systems for electron microscopic analyses -- Fixation and embedding -- Preparation of colloidal gold -- Conjugation of colloidal gold to proteins -- Colloidal gold/streptavidin methods -- Pre-embedding labeling methods -- Postembedding labeling methods -- Photography -- Overview of fluorescence photomicrography -- Special applications -- Overview of fluorescence analysis with the confocal microscope -- Overview of laser microbeam applications as related to antibody targeting -- Nucleic acid immunocytochemistry : in situ hybridization to human chromosomes of an alkaline phosphatase-labeled centromeric probe -- Fluorescence in situ hybridization using whole chromosome library probes -- Overview of automated immunostainers -- Overview of immunocytochemical approaches to the differential diagnosis of tumors: diagnostic applications in the clinical laboratory
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592595211&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357193'}
Extent
1 online resource (xii, 435 pages)
File format
multiple file formats
Form of item
online
Isbn
9781592595211
Lccn
94022475
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote

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