Coverart for item
The Resource Hydrocarbon and lipid microbiology protocols : microbial quantitation, community profiling and array approaches, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Hydrocarbon and lipid microbiology protocols : microbial quantitation, community profiling and array approaches, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Label
Hydrocarbon and lipid microbiology protocols : microbial quantitation, community profiling and array approaches
Title
Hydrocarbon and lipid microbiology protocols
Title remainder
microbial quantitation, community profiling and array approaches
Statement of responsibility
Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Contributor
Editor
Subject
Genre
Language
eng
Summary
This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
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Cataloging source
RML
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Springer protocols handbooks,
Hydrocarbon and lipid microbiology protocols : microbial quantitation, community profiling and array approaches, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Label
Hydrocarbon and lipid microbiology protocols : microbial quantitation, community profiling and array approaches, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-3-662-52778-8
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities
  • Boyd A. McKew and Cindy J. Smith
  • Microbial biomass and community composition analysis using phospholipid fatty acids
  • Aaron D. Peacock and David C. White
  • Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis
  • Stefan J. Green, Mary Beth Leigh, and Josh D. Neufeld
  • Microbial community profiling : SSCP and T-RFLP techniques
  • Christoph C. Tebbe, Anja B. Dohrmann, Michael Hemkemeyer, and Astrid N€ather
  • Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities
  • Mary Beth Leigh, Lee Taylor, and Josh D. Neufeld
  • Introduction to microbial quantitation, community profiling, and array approaches
  • Microbial community analysis by single-amplicon high-throughput next generation sequencing : data analysis - from raw output to ecology
  • Alex J. Dumbrell, Robert M.W. Ferguson, and Dave R. Clark
  • Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment
  • Yolanda Blanco, Mercedes Moreno-Paz, Jacobo Aguirre, and Victor Parro
  • Studying protistan communities in hydrocarbon-contaminated environments
  • Julia Johnke and Antonis Chatzinotas
  • Josh D. Neufeld
  • Cell extraction method for oily sediments
  • Michael Lappe and Jens Kallmeyer
  • Introduction to microplate MPN enumeration of hydrocarbon degraders
  • Anders R. Johnsen
  • Primers for dsr genes and most probable number method for detection of sulfate-reducing bacteria in oil reservoirs
  • Yin Shen and Gerrit Voordouw
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9783662527764&userID=ebsco-test&password=ebsco-test
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unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b4160394'}
Extent
1 online resource (x, 252 pages)
File format
unknown
Form of item
online
Isbn
9783662527764
Lccn
2016938230
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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