Coverart for item
The Resource Hydrocarbon and lipid microbiology protocols : genetic, genomic and system analyses of communities, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Hydrocarbon and lipid microbiology protocols : genetic, genomic and system analyses of communities, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Label
Hydrocarbon and lipid microbiology protocols : genetic, genomic and system analyses of communities
Title
Hydrocarbon and lipid microbiology protocols
Title remainder
genetic, genomic and system analyses of communities
Statement of responsibility
Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Contributor
Editor
Subject
Genre
Language
eng
Summary
This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
Member of
Cataloging source
RML
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Springer protocols handbooks,
Hydrocarbon and lipid microbiology protocols : genetic, genomic and system analyses of communities, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Label
Hydrocarbon and lipid microbiology protocols : genetic, genomic and system analyses of communities, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-3-662-50450-5
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Prokaryotic metatranscriptomics
  • Danilo Perez-Pantoja and Javier Tamames
  • Defining a pipeline for metaproteomic analyses
  • Joseph A. Christie-Oleza, Despoina Sousoni, Jean Armengaud, Elizabeth M. Wellington, and Alexandra M.E. Jones
  • Metabolic profiling and metabolomic procedures for investigating the biodegradation of hydrocarbons
  • Vincent Bonifay, Egemen Aydin, Deniz F. Aktas, Jan Sunner, and Joseph M. Suflita
  • Generating enriched metagenomes from active microorganisms with DNA stable isotope probing
  • Carolina Grob, Martin Taubert, Alexandra M. Howat, Oliver J. Burns, Yin Chen, Josh D. Neufeld, and J. Colin Murrell
  • DNA- and RNA-based stable isotope probing of hydrocarbon degraders
  • Tillmann Lueders
  • Introduction to genetic, genomic, and system analyses for communities
  • Protocol for performing protein stable isotope probing (protein-SIP) experiments
  • Nico Jehmlich and Martin von Bergen
  • Protein extraction from contaminated soils and sediments
  • Mercedes V. Del Pozo, Monica Martınez-Martınez, and Manuel Ferrer
  • Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities
  • Wilfred F.M. Roling, Lucas Fillinger, and Ulisses Nunes da Rocha
  • Constructing and analyzing metabolic flux models of microbial communities
  • Jose P. Faria, Tahmineh Khazaei, Janaka N. Edirisinghe, Pamela Weisenhorn, Samuel M.D. Seaver, Neal Conrad, Nomi Harris, Matthew DeJongh, and Christopher S. Henry
  • Protocol for evaluating the permissiveness of bacterial communities toward conjugal plasmids by quantification and isolation of transconjugants
  • Uli Klumper, Arnaud Dechesne, and Barth F. Smets
  • Jack A. Gilbert and Nicole M. Scott
  • Genomic analysis of pure cultures and communities
  • Stepan V. Toshchakov, Ilya V. Kublanov, Enzo Messina, Michail M. Yakimov, and Peter N. Golyshin
  • Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems
  • Stephanie M. Moormann, Jarrad T. Hampton-Marcell, Sarah M. Owens, and Jack A. Gilbert
  • Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems
  • Jarrad T. Hampton-Marcell, Angel Frazier, Stephanie M. Moormann, Sarah M. Owens, and Jack A. Gilbert
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9783662504499&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b4160395'}
Extent
1 online resource (x, 288 pages)
File format
unknown
Form of item
online
Isbn
9783662504499
Lccn
2016938230
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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