Coverart for item
The Resource Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Label
Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods
Title
Hydrocarbon and lipid microbiology protocols
Title remainder
single-cell and single-molecule methods
Statement of responsibility
Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Contributor
Editor
Subject
Genre
Language
eng
Summary
Annotation
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Springer Protocols handbooks,
Summary expansion
This volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy
Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Label
Hydrocarbon and lipid microbiology protocols : single-cell and single-molecule methods, Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-3-662-49131-7
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction: Single cell biotechnology for uncultured microorganisms -- Single-cell cultivation in microfluidic devices -- Single-cell bacterial bioreporter assays to measure hydrocarbons -- Whole genome amplification of single bacterial cells -- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria -- Localisation of proteins within intact bacterial cells using fluorescent protein fusions -- Raman-FISH -- Single-cell metabolomics -- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division -- Single cell biotechnology for uncultured microorganisms -- Single cell and systems biology tools for biofuel production -- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) -- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity -- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9783662491317&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3793156'}
Extent
1 online resource (x, 204 pages)
File format
unknown
Form of item
online
Isbn
9783662491317
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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