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The Resource Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz, (electronic resource)

Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz, (electronic resource)

Label
Human stem cell manual : a laboratory guide
Title
Human stem cell manual
Title remainder
a laboratory guide
Statement of responsibility
edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz
Contributor
Subject
Genre
Language
eng
Summary
Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses
Cataloging source
OPELS
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz, (electronic resource)
Label
Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz, (electronic resource)
Link
http://libproxy.rpi.edu/login?url=http://www.sciencedirect.com/science/book/9780123704658
Publication
Related Contributor
Related Location
Related Agents
Related Authorities
Related Subjects
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
  • Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1.Human Embryonic Stem Cell Culture -- 2.Feeder layers and feeder-free culture -- 3.Mouse Embryonic Fibroblast Feeder Cells -- 4.Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5.Classical Cytogenetics: Karyotyping -- 6.Spectral Karyotyping and Fluorescent in situ Hybridization -- 7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8.FACS/Cell sorting -- 9.Fluorescence Immunocytochemical Analysis of Stem Cells -- 10.Characterization of Stem Cells Using RT-PCR -- 11.Gene Expression Profiling of Stem Cells by Microarray -- 12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13.Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14.Embryoid Body Formation and Neuroepithelia Differentiation -- 15.Motor Neuron and Dopamine Neuron Differentiation -- 16.Oligodendrocyte Differentiation from hESC -- 17.Cardiac -- 18.Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19.Genetic Manipulation of hESC: Lentivirus Vectors -- 20.Methods developed for mouse ES cells -- Part V: Advanced methods -- 21.Derivation of human ES cells -- 22.Neural Stem Cell Culture -- 23.Transplantation of stem cells -- 24.In vitro fertilization -- 25.Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26.Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27.Intellectual Property: Owning the Stem Cell -- 28.Ethical Concerns for Stem Cell Research -- 29.Guidelines for hESC Research Oversight (ESCRO) Committees
  • Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9780123704658&userID=ebsco-test&password=ebsco-test
Dimensions
26 cm.
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b2400671'}
Extent
xxiii, 461 p.
Form of item
electronic
Isbn
9780123704658
Other physical details
ill. (some col.)
Reproduction note
Electronic reproduction.
Specific material designation
remote

Library Locations

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      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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