Coverart for item
The Resource Human retrovirus protocols : virology and molecular biology, edited by Tuofu Zhu

Human retrovirus protocols : virology and molecular biology, edited by Tuofu Zhu

Label
Human retrovirus protocols : virology and molecular biology
Title
Human retrovirus protocols
Title remainder
virology and molecular biology
Statement of responsibility
edited by Tuofu Zhu
Contributor
Subject
Genre
Language
eng
Summary
This book is a cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources.; There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 304
Human retrovirus protocols : virology and molecular biology, edited by Tuofu Zhu
Label
Human retrovirus protocols : virology and molecular biology, edited by Tuofu Zhu
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592599079
Publication
Related Contributor
Related Location
Related Agents
Related Authorities
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Enhanced culture assay for detection and quantitation of latently infected, resting cd4+ t-cells carrying replication-competent virus in HIV-1-infected individuals / J.D. Siliciano and R.F. Siliciano -- Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals / H. Schuitemaker and N.A. Kootstra -- Isolation of human immunodeficiency virus type 1 from peripheral blood monocytes / P.R. Gorry [and others] -- Isolation, propagation, and HIV-1 infection of monocyte-derived macrophages and recovery of virus from brain and cerebrospinal fluid / S. Gorantla, M. Che and H.E. Gendelman -- Isolation and HIV-1 infection of primary human microglia from fetal and adult tissue / K. Borgmann, H.E. Gendelman and A. Ghorpade -- Isolation of human immunodeficiency virus type 1 from semen and vaginal fluids / A.A. Kiessling -- Isolation and quantification of HIV from lymph nodes / C. Tamalet -- Isolation of human immunodeficiency virus type 2 biological clones from peripheral blood lymphocytes / H. Blaak -- Isolation and confirmation of human t-cell leukemia virus type 2 from peripheral blood mononuclear cells / M.D. Lairmore and A. Montgomery -- Isolation of foamy viruses from peripheral blood lymphocytes / J. Tobaly-Tapiero, P. Bittoun and A. Saib -- Alu-ltr real-time nested PCR assay for quantifying integrated HIV-1 DNA / A. Brussel, O. Delelis and P. Sonigo -- Quantification of hfv-integrated DNA in human cells by alu-ltr real-time PCR / O. Delelis, A. Brussel and P. Sonigo -- Detection of HIV-1 provirus and rna by in situ amplification / A. Nicol and G.J. Nuovo -- Detection of htlv-1 gene on cytologic smear slides / K. Kashima, T. Daa and S. Yokoyama -- Detection of HIV-2 by PCR / F. Gao -- Quantitation of HIV-1 viral rna in blood plasma and genital secretions / S.A. Fiscus -- Quantification of proviral DNA load of human immunodeficiency virus type 2 subtypes a and b using real-time PCR / M. Gueudin, F. Damond and F. Simon -- Plasma rna viral load in HIV-1 group o infection by real-time PCR / M. Gueudin and F. Simon -- A new combined HIV p24 antigen and anti-HIV-1/2/o screening assay / S. Polywka [and others] -- A new automated fourth-generation HIV screening assay with sensitive antigen detection module and high specificity / B. Weber -- Detection of antiviral antibodies using enzyme-linked immunosorbent assay / I.G. Winkler -- Plaque-reduction assays for human and simian immunodeficiency virus neutralization / A. Nordqvist and E.M. Fenyo -- Detection of drug-resistant HIV-1 strains / A.A. Kiessling, S.J. Eyre and B.D. Desmarais -- Determination of cell tropism of HIV-1 / N.A. Kootstra and H. Schuitemaker -- Determination of co-receptor usage of HIV-1 / H. Schuitemaker and N.A. Kootstra -- Quantitative evaluation of HIV and SIV co-receptor use with ghost(3) cell assay / D. Vodros and E.M. Fenyo -- Phenotypic characterization of blood monocytes from HIV-infected individuals / P.J. Ellery and S.M. Crowe -- Methods to determine HIV-1 ex vivo fitness / A. Abraha [and others] -- A yeast recombination-based cloning system to produce chimeric HIV-1 viruses and express HIV-1 genes / D.M. Moore [and others] -- PCRs amplification, cloning, and construction of HIV-1 infectious molecular clones from virtually full-length HIV-1 genomes / P.K. Ehrenberg and N.L. Michael -- Amplification and cloning of near full-length HIV-2 genomes / F. Gao -- Growth and manipulation of a human t-cell leukemia virus type 2 full-length molecular clone / M. Anderson and P.L. Green -- Construction and analysis of genomic, full-length infectious foamy virus DNA clones / R. Wirtz and M. Lochelt -- Molecular characterization of proteolytic processing of the gag proteins of human spumaretrovirus / K.I. Pfrepper and R.M. Flugel -- Assessing the relative efficacy of antiretroviral activity of different drugs on macrophages / S. Aquaro and C.F. Perno -- Gene expression profiling of HIV-1 infection using cdna microarrays / A.B. van 't Wout -- Determination of DC-sign and DC-signr repeat region variations / H. Liu and T. Zhu
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781588294951&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356918'}
Extent
1 online resource (xiv, 494 pages)
Form of item
online
Isbn
9781588294951
Lccn
2004026213
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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