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The Resource Human embryonic stem cell protocols, edited by Kursad Turksen

Human embryonic stem cell protocols, edited by Kursad Turksen

Human embryonic stem cell protocols
Human embryonic stem cell protocols
Statement of responsibility
edited by Kursad Turksen
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
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index present
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology,
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Human embryonic stem cell protocols, edited by Kursad Turksen
Human embryonic stem cell protocols, edited by Kursad Turksen
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Includes bibliographical references and index
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online resource
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Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis -- Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov -- Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen Escobedo-Lucea and miodrag Stojkovic -- In vitro neural differentiation of human embronic stem cells using a low-density mouse embryonic fibroblast feeder protocol / John A. Ozolek [and others] -- Optimization of physiological xenofree molecularly defined media and matrices to maintain human embryonic stem cell pluripotency / Isabelle Pfeiffer [and others] -- Serum-free and feeder-free culture expansion of human embryonic stem cells / Katherine E. Wagner and Mohan C. Vemuri -- Single cell enzymatic dissociation of human embryonic stem cells : a straightforward, robust, and standardized culture method / Catharina Ellerström, Johan Hyllner, and Raimund Strehl -- Monitoring stemness in long-term hESC cultures by real-time PCR / Amparo Galán and Carlos Simón -- Culture and preparation of human embryonic stem cells for proteomics-based applications / Charles C. King -- A two- and three-dimensional approach for visualizing human embryonic stem cell differentiation / Christian B. Brøchner [and others] -- Immunofluorescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions / Aashir Awan [and others] -- Study of gap junctions in human embryonic stem cells / Raymond C.B. Wong and Alice Pébay -- hESC engineering by integrase-mediated chromosomal targeting / Ying Liu [and others] -- Human embryonic stem cell differentiation on periodontal ligament fibroblasts / Y. Murat Elc̦in, Bülend İnanc̦, and A. Eser Elc̦in -- Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells / Ronald S. Goldstein [and others] -- Embryonic stem cells as a model for studying melanocyte development / Susan E. Zabierowski and Meenhard Herlyn -- In vitro derivation of chondrogenic cells from human embryonic stem cells / Wei Seong Toh [and others] -- Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds / Sharon Gerecht, Lino S. Ferreira, and Robert Langer -- Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells / Xiao-Quing Zhang and Su-Chung Zhang -- Transplantation of human embryonic stem cells and derivatives to the chick embryo / Ronald S. Goldstein -- Genetic manipulation of human embryonic stem cells / Silvina Epsztejn-Litman and Rachel Eiges -- Genetic manipulation of human embryonic stem cells in serum and feeder-free media / Stefan R. Braam, Chris Denning, and Christine L. Mummery -- Human-induced pluripotent stem cells : derivation, propagation, and freezing in serum- and feeder layer-free culture conditions / Hossein Baharvand [and others]
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2nd ed.
1 online resource (xii, 455 pages)
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illustrations (some color).
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Electronic reproduction.
unknown sound
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Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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