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The Resource Heterologous protein production in CHO cells : methods and protocols, edited by Paula Meleady

Heterologous protein production in CHO cells : methods and protocols, edited by Paula Meleady

Label
Heterologous protein production in CHO cells : methods and protocols
Title
Heterologous protein production in CHO cells
Title remainder
methods and protocols
Statement of responsibility
edited by Paula Meleady
Contributor
Editor
Subject
Genre
Language
eng
Summary
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
volume 1603
Heterologous protein production in CHO cells : methods and protocols, edited by Paula Meleady
Label
Heterologous protein production in CHO cells : methods and protocols, edited by Paula Meleady
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-4939-6972-2
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Strategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells -- Glycoengineering of CHO Cells to Improve Product Quality -- Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension -- Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression -- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells -- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors -- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells -- Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture -- Selection of High-Producing Clones using FACS for CHO Cell Line Development -- The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity -- A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data -- Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells -- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells -- Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture -- Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells -- Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses
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https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781493969722&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b4373182'}
Extent
1 online resource (x, 252 pages)
File format
unknown
Form of item
online
Isbn
9781493969722
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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