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The Resource Hemostasis and thrombosis protocols, edited by David J. Perry and K. John Pasi, (electronic resource)

Hemostasis and thrombosis protocols, edited by David J. Perry and K. John Pasi, (electronic resource)

Hemostasis and thrombosis protocols
Hemostasis and thrombosis protocols
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edited by David J. Perry and K. John Pasi
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index present
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non fiction
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  • standards specifications
  • bibliography
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Methods in molecular medicine
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Hemostasis and thrombosis protocols, edited by David J. Perry and K. John Pasi, (electronic resource)
Hemostasis and thrombosis protocols, edited by David J. Perry and K. John Pasi, (electronic resource)
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Bibliography note
Includes bibliographical references and index
pt. 1. Introduction -- Hemostasis: components and processes / K. John Pasi -- pt. 2. Basic techniques -- Isolation of DNA and RNA / David J. Perry -- Amplification of DNA and RNA by PCR / David J. Perry -- Direct sequencing of PCR products / David J. Perry -- Solid-phase sequencing of biotinylated PCR products with streptavidin-coated magnetic beads / David J. Perry -- Automated DNA sequencing / Helen L. Devereux -- Detection of DNA by silver staining / David J. Perry -- Promoter studies in hemostasis / Peter R. Winship -- pt. 3. Methods of mutational analysis -- Detection of mutations and polymorphisms in clotting factors by denaturing gradient gel electrophoresis / Rainer Schwaab -- Screening for mutations in DNA by single-stranded conformation polymorphism (SSCP) analysis / David J. Perry -- Screening for DNA heteroduplexes in the factor VII gene using ethylene glycol gel electrophoresis of solvent-treated 32P-labeled PCR products / Peter M. Baker -- Detection of mutations causing hemophilia A using in vitro coupled transcription and translation system / Chike Ononye -- Screening for mutations in the human antithrombin gene by Hydrolink D-5000 and MDE gel electrophoresis / David J. Perry -- pt. 4. Methods for analyzing inherited/acquired disorders of hemostasis -- Detection of mutations in hemophilia A patients by chemical cleavage of mismatch method / Naushin H. Waseem -- Inversion mutation analysis in hemophilia A by restriction enzyme analysis and southern blotting / Chike Ononye -- Hemophilia B mutational analysis / Peter M. Green -- Screening for candidate mutations causing von Willebrand's Disease (vWD) / P. Vincent Jenkins -- Use of intron 40 VNTR I in vWD gene tracking / Mohammed S. Enayat -- Mutlimeric analysis of von Willebrand Factor (vWF) / Mohammed S. Enayat -- Identification of mutations in the human factor VII gene / Peter M. Baker -- Molecular analysis in factor XI deficiency / Karen M. Johnson -- Mutational analysis in antithrombin deficiency / David J. Perry -- Ectopic transcript analysis in human antithrombin deficiency / David J. Perry -- Mutational analysis of the human protein C gene / Roger Luddington -- Analysis of the protein S gene in protein S deficiency / Núria Sala -- Screening for the G to A transition at position 20210 in the 3'-untranslated region (UTR) of the prothrombin gene / Karen P. Brown -- Screening for the factor V leiden mutation / Karen P. Brown -- Multiplex PCR for detection of the prothrombin 3' UTR (C20210A) polymorphism and the factor V leiden mutation / Gillian Mellars -- Isoelectric focusing and immunodetection of plasma antithrombin / Martina Daly -- Characterization of heparin binding variants of antithrombin by crossed immunoelectrophoresis in the presence of heparin / Martina Daly -- The determination of amino acid sequence abnormalities in proteins by HPLC peptide analysis / David Williamson -- pt. 5. Platelet and megakaryocyte analysis -- Molecular biological identification and characterization of inherited platelet receptor disorders / Ramesh B. Basani -- In vitro expansion of megakaryocytes from peripheral blood hematopoietic progenitors / Michael A. Thornton -- Molecular biology studies with primary megakaryocytes / Yaping Shou
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1 online resource (xiii, 368 p.)
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