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The Resource Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny

Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny

Handbook of ELISPOT : methods and protocols
Handbook of ELISPOT
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methods and protocols
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edited by Alexander E. Kalyuzhny
Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use
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non fiction
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  • dictionaries
  • bibliography
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Methods in molecular biology
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Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny
Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny
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Includes bibliographical references and index
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online resource
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  • The ELISPOT assay : a personal retrospective / Jonathan Sedgwick -- Chemistry and biology of the ELISPOT assay / Alexander E. Kalyuzhny -- Membranes and membrane plates used in ELISPOT / Alan J. Weiss -- Standardization and validation issues of the ELISPOT assay / Sylvia Janetzki [and others] -- A cell detachment solution can reduce a background staining in ELISPOT assay : Accumax / Angela Grant [and others] -- Isolation of subsets of immune cells / Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves -- Image analysis and data management of ELISPOT assay results / Paul Viktor Lehmann -- High resolution as a key feature to perform accurate ELISPOT measurements using Zeiss KS ELISPOT readers / Wolf Malkusch
  • Improving the sensitivity of the ELISPOT analyses of antigen-specific cellular immune responses in rhesus macaques / K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete -- Feline cytokine ELISPOT : issues in assay development / Sushila K. Nordone [and others] -- Measuring T cell function in animal models of tuberculosis by ELISPOT / Vanja Lazarevic, Santosh Pawar, and JoAnne Flynn -- IFN-gamma ELISPOT assay for the quantitative measurement of antigen-specific murine CD8+ T cells / Geoffrey A. Cole -- Detection of measles virus-specific IFN-gamma secreting T cells by ELISPOT / Jenna E. Ryan, Inna G. Ovsyannikova, and Gregory A. Poland -- Epitope mapping in multiple sclerosis using the ELISPOT assay / Clara M. Pelfrey and Ioana R. Moldovan
  • Use of IFN-gamma ELISPOT in monitoring immune responses in humans / Mark Matijevic and Robert G. Urban -- ELISPOT determination of IFN-gamma T cell frequencies in patients with autoimmune sensorineural hearing loss / C. Arturo Solares and Vincent K. Tuohy -- Dual color ELISPOT assay for analyzing cytokine balance / Yoshihiro Okamoto and Mikio Nishida -- Simultaneous detection of multiple cytokines in ELISPOT assays / Sarah Palzer [and others] -- Fluorospot assay : methodological analysis / Agnes Gazagne [and others] -- A gel-based dual antibody capture and detection method for assaying of extracellular cytokine secretion : EliCell / Lisa A. Spencer [and others]
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1 online resource (xiv, 321 pages)
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