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The Resource Formalin-fixed paraffin-embedded tissues : methods and protocols, edited by Fahd Al-Mulla

Formalin-fixed paraffin-embedded tissues : methods and protocols, edited by Fahd Al-Mulla

Label
Formalin-fixed paraffin-embedded tissues : methods and protocols
Title
Formalin-fixed paraffin-embedded tissues
Title remainder
methods and protocols
Statement of responsibility
edited by Fahd Al-Mulla
Contributor
Subject
Language
eng
Summary
Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
724
Formalin-fixed paraffin-embedded tissues : methods and protocols, edited by Fahd Al-Mulla
Label
Formalin-fixed paraffin-embedded tissues : methods and protocols, edited by Fahd Al-Mulla
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-055-3
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Microwaves for chromogenic In situ hybridization
  • Anthony S.-Y. Leong and Zenobia Haffajee
  • Automated analysis of FISH-stained HER2/neu samples with metafer
  • Christian Schunck and Eiman Mohammad
  • Laser capture microdissection of FFPE tissue sections bridging the gap between microscopy and molecular analysis
  • Renate Burgemeister
  • Nucleic acids extraction from laser microdissected FFPE tissue sections
  • Renate Burgemeister
  • Microarray-based CGH and copy number analysis of FFPE samples
  • Fahd Al-Mulla
  • Regulatory and ethical Issues on the utilization of FFPE tissues in research
  • Microarray profiling of DNA extracted from FFPE tissues using SNP 6.0 affymetrix platform
  • Marianne Tuefferd [and others]
  • Whole genome amplification of DNA extracted from FFPE tissues
  • Mira Bosso and Fahd Al-Mulla
  • Pyrosequencing of DNA extracted from formalin-fixed paraffin-embedded tissue
  • Brendan Doyle, Ciaran O'Riain, and Kim Appleton
  • Analysis of DNA methylation in FFPE tissues using the methylight technology
  • Ashraf Dallol [and others]
  • RT-PCR gene expression profiling of RNA from paraffin-embedded tissues prepared using a range of different fixatives and conditions
  • Mei-Lan Liu [and others]
  • Catherine M. With, David L. Evers, and Jeffrey T. Mason
  • RT-PCR-based gene expression profiling for cancer biomarker discovery from fixed, paraffin-embedded tissues
  • Aaron Scott [and others]
  • MicroRNA isolation from formalin-fixed, paraffin-embedded tissues
  • Aihua Liu and Xiaowei Xu
  • Gene expression profiling of RNA extracted from FFPE tissues : NuGEN technologies' whole-transcriptome amplification system
  • Leah Turner, Joe Don Heath, and Nurith Kurn
  • Protein mass spectrometry applications on FFPE tissue sections
  • Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason
  • Alternative fixative to formalin fixation for molecular applications : the RCL2®-CS100 approach
  • Amelie Denouel [and others]
  • Tissue microarrays : construction and uses
  • Carol B. Fowler [and others]
  • Standardization in immunohistology
  • Anthony S.-Y. Leong and Trishe Y.-M. Leong
  • Multiple immunofluorescence labeling of formalin-fixed paraffin-embedded tissue
  • David Robertson and Clare M. Isacke
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617790553&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357626'}
Extent
1 online resource (xii, 312 pages)
Form of item
online
Isbn
9781617790553
Lccn
2011922256
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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