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The Resource Epstein-Barr virus protocols, edited by Joanna B. Wilson and Gerhard H.W. May

Epstein-Barr virus protocols, edited by Joanna B. Wilson and Gerhard H.W. May

Label
Epstein-Barr virus protocols
Title
Epstein-Barr virus protocols
Statement of responsibility
edited by Joanna B. Wilson and Gerhard H.W. May
Contributor
Subject
Genre
Language
eng
Summary
The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • handbooks
Series statement
Methods in molecular biology
Series volume
174
Epstein-Barr virus protocols, edited by Joanna B. Wilson and Gerhard H.W. May
Label
Epstein-Barr virus protocols, edited by Joanna B. Wilson and Gerhard H.W. May
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592592279
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Genome and transcript analyses
  • Viral detection
  • Culture methods
  • Immune assays
  • Viral protein detection
  • Protein-protein, protein-DNA and protein-RNA interactions
  • Protein activity assays
  • Tissue and in vivo protocols
  • Epstein-Barr virus: the B95-8 strain map -- Analysis of replication of oriP-based plasmids by quantitative, competitive PCR -- Granulocyte and granulocyte macrophage colony-stimulating factors as therapy in human and veterinary medicine -- Analysis of the expression and function of the EBV-encoded small RNAs, the EBERs, in heterologous cells -- Prevention of hepatocellular carcinoma in chronic viral hepatitis patients with cirrhosis by carotenoid mixture -- In situ detection of Epstein-Barr virus DNA and viral gene products -- Detection of EBV infection at the single-cell level. Precise quantitation of virus-infected cells in vivo -- The effects of pH on DNA methylation state: In vitro and post-mortem brain studies -- Autolink: Automated sequential resonance assignment of biopolymers from NMR data by relative-hypothesis-prioritization-based simulated logic -- Introduction of plasmid vectors into cells via electroporation -- The maize AB10 meiotic drive system maps to supernumerary sequences in a large complex haplotype -- Hemozoin-free plasmodium falciparum mitochondria for physiological and drug susceptibility studies -- Seasonal and spatial community dynamics in the meromictic Lake Cadagno -- Use of fluorescent dyes as molecular probes for the study of multidrug resistance -- Qualitative detection of apoptotic cells assessed by DNA fragmentation -- A novel method of labeling and characterizing migrating neurons in the developing central nervous system -- Effect of chronic ethanol ingestion on [alpha]-mannosidase isoenzymes in rat liver -- Pulmonary vascular endothelial growth factor-C in development and lung injury in preterm infants -- Comparative evaluation of positive tests to Mycobacterium avium subsp. Paratuberculosis in clinically healthy sheep and goats in south-west Greece using molecular techniques, serology, and culture -- Heritable endosymbionts of drosophila -- Genetic mapping in a natural population of collared flycatchers (Ficedula albicollis): Conserved synteny but gene order rearrangements on the avian Z chromosome -- Utilization of polymerase chain reaction and selective media cultivation to identify legionella in Taiwan spring water samples -- The genetic basis of head and neck carcinoma -- Multiple uses of liquepipets in southern, northern, and western blots -- Pharmacogenetics of antipsychotic-induced weight gain -- From imaging to understanding: Frontiers in live cell imaging, Bethesda, MD, April 19-21, 2006 -- Accumulation of recessive lethal mutations in saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements -- Pkcalpha: A versatile key for decoding the cellular calcium toolkit -- An efficient genetic screen in drosophila to identify nuclear-encoded genes with mitochondrial function -- Pharmacogenetic studies of alcohol self-administration and withdrawal -- Thomas G. Orr memorial lectureship. Colon cancer from etiology to prevention -- Outbreak of trichinellosis associated with eating cougar jerky -- In vivo models of prostate cancer metastasis to bone -- Variable populations within variable populations: Quantifying mitochondrial heteroplasmy in natural populations of the gynodioecious plant silene vulgaris -- Enrichment of fetal trophoblast cells from the maternal peripheral blood followed by detection of fetal deoxyribonucleic acid with a nested X/Y polymerase chain reaction -- Real-time FRET PCR assay for salmonella enterica serotype detection in food -- Large-scale gene expression differences across brain regions and inbred strains correlate with a behavioral phenotype -- Malate dehydrogenase from chlorobium vibrioforme, chlorobium tepidum, and heliobacterium gestii: Purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis -- Long-term persistence of ciprofloxacin-resistant haemophilus influenzae in patients with cystic fibrosis -- Isolation and comparison of the paracrystalline surface layer proteins of freshwater caulobacters -- Purification and partial characterization of transglutaminase from physarum polycephalum -- An operon of bacillus subtilis motility genes transcribed by the sigma D form of RNA polymerase -- Physical mapping of repetitive extragenic palindromic sequences in escherichia coli and phylogenetic distribution among escherichia coli strains and other enteric bacteria -- Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: Implications for allograft rejection -- [Gamma] [delta] T cells respond directly to pathogen-associated molecular patterns -- Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes PCBAB, PCBC, and pende are clustered on chromosome VI (3.0 megabases) -- In silico identification of supertypes for class II MHCS -- Cloning of a metalloprotease gene involved in the virulence mechanism of vibrio anguillarum -- The lethal phenotype caused by null mutations in the escherichia coli HTRB gene is suppressed by mutations in the ACCBC operon, encoding two subunits of acetyl coenzyme a carboxylase
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Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356722'}
Extent
1 online resource (xiv, 438 pages)
Form of item
online
Isbn
9781592592272
Lccn
2001016991
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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