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The Resource Enzyme engineering : methods and protocols, edited by James C. Samuelson

Enzyme engineering : methods and protocols, edited by James C. Samuelson

Enzyme engineering : methods and protocols
Enzyme engineering
Title remainder
methods and protocols
Statement of responsibility
edited by James C. Samuelson
Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs
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index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
v. 978
Enzyme engineering : methods and protocols, edited by James C. Samuelson
Enzyme engineering : methods and protocols, edited by James C. Samuelson
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Bibliography note
Includes bibliographical references and index
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online resource
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  • TAL effector nuclease (TALEN) engineering
  • Ting Li and Bing Yang
  • In vitro evolution of enzymes
  • Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli
  • Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
  • Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
  • Reconstructing evolutionary adaptive paths for protein engineering
  • Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
  • Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
  • Bryan M. Swingle
  • A Tripartite fusion system for the selection of protein variants with increased stability in vivo
  • FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
  • Eric R. Geertsma
  • Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
  • Hidehiko Hirakawa and Teruyuki Nagamune
  • Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
  • Romualdas Vaisvila and Jurate Bitinaite
  • Protein engineering : single or multiple site-directed mutagenesis
  • Pei-Chung Hsieh and Romualdas Vaisvila
  • Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
  • Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
  • Linda Foit and James C.A. Bardwell
  • Promiscuity-based enzyme selection for rational directed evolution experiments
  • Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar
  • Rational protein sequence diversification by multi-codon scanning mutagenesis
  • Jia Liu and T. Ashton Cropp
  • Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
  • Jian-Wei Liu and David L. Ollis
  • In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
  • Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis
  • Determining enzyme kinetics via isothermal titration calorimetry
  • Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
  • GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
  • Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo
  • Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
  • Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg
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1 online resource (xi, 252 pages)
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illustrations (some color).
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