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The Resource Enzyme engineering : methods and protocols, edited by James C. Samuelson

Enzyme engineering : methods and protocols, edited by James C. Samuelson

Label
Enzyme engineering : methods and protocols
Title
Enzyme engineering
Title remainder
methods and protocols
Statement of responsibility
edited by James C. Samuelson
Contributor
Subject
Genre
Language
eng
Summary
Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs
Member of
Cataloging source
SCB
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
v. 978
Enzyme engineering : methods and protocols, edited by James C. Samuelson
Label
Enzyme engineering : methods and protocols, edited by James C. Samuelson
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-62703-293-3
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • TAL effector nuclease (TALEN) engineering
  • Ting Li and Bing Yang
  • In vitro evolution of enzymes
  • Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli
  • Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
  • Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
  • Reconstructing evolutionary adaptive paths for protein engineering
  • Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
  • Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
  • Bryan M. Swingle
  • A Tripartite fusion system for the selection of protein variants with increased stability in vivo
  • FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
  • Eric R. Geertsma
  • Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
  • Hidehiko Hirakawa and Teruyuki Nagamune
  • Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
  • Romualdas Vaisvila and Jurate Bitinaite
  • Protein engineering : single or multiple site-directed mutagenesis
  • Pei-Chung Hsieh and Romualdas Vaisvila
  • Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
  • Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
  • Linda Foit and James C.A. Bardwell
  • Promiscuity-based enzyme selection for rational directed evolution experiments
  • Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar
  • Rational protein sequence diversification by multi-codon scanning mutagenesis
  • Jia Liu and T. Ashton Cropp
  • Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
  • Jian-Wei Liu and David L. Ollis
  • In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
  • Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis
  • Determining enzyme kinetics via isothermal titration calorimetry
  • Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
  • GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
  • Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo
  • Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
  • Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg
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Extent
1 online resource (xi, 252 pages)
Form of item
online
Isbn
9781627032933
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote

Library Locations

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      42.729766 -73.682577
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