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The Resource Embryonic stem cells : methods and protocols, edited by Kursad Turksen

Embryonic stem cells : methods and protocols, edited by Kursad Turksen

Embryonic stem cells : methods and protocols
Embryonic stem cells
Title remainder
methods and protocols
Statement of responsibility
edited by Kursad Turksen
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index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
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Summary expansion
Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions
Embryonic stem cells : methods and protocols, edited by Kursad Turksen
Embryonic stem cells : methods and protocols, edited by Kursad Turksen
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Includes bibliographical references and index
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online resource
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Machine generated contents note: 1 Methods for the Isolation and Maintenance of Murine Embryonic -- Stem Cells -- Marsha L. Roach and John D. McNeish1 -- 2 The Use of a Chemically Defined Media for the Analyses of Early -- Development in ES Cells and Mouse Embryos -- Gabriele Proetzel and Michael V. Wiles17 -- 3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells -- Pierre Savatier, H6elne Lapillonne, Ludmila Jirmanova, -- Luigi Vitelli, and Jacques Samarut27 -- 4 Murine Embryonic Stem Cells as a Model for Stress Proteins -- and Apoptosis During Differentiation -- Andre-Patrick Arrigo and Patrick Mehlen35 -- 5 Effects of Altered Gene Expression on ES Cell Differentiation -- Yong Fan and J. Richard Chaillet45 -- 6 Hypoxic Gene Regulation in Differentiating ES Cells -- David M. Adelman and M. Celeste Simon55 -- 7 Regulation of Gap Junction Protein (Connexin) Genes and Function -- in Differentiating ES Cells -- Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, -- and Tetsuro Takam atsu63 -- 8 Embryonic Stem Cell Differentiation as a Model to Study -- Hematopoietic and Endothelial Cell Development -- Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, -- and Shin-lchi N ishikaw a71 -- 9 Analysis of Bcr-AblFunction Using an In Vitro Embryonic Stem Cell -- Differentiation System -- Takumi Era, Stephane Wong, and Owen N. Witte 83 -- 10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage -- Development -- Toshiyuki Yamane, Takahiro Kunisada, and Shin-lchi Hayashi 97 -- 11 Differentiation of Embryonic Stem Cells as a Model to Study Gene -- Function During the Development of Adipose Cells -- C hristian D ani107 -- 12 Embryonic Stem Cell Differentiation and the Vascular Lineage -- V ictoria L.B autch117 -- 13 Embryonic Stem Cells as a Model to Study Cardiac, -- Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiatiin -- Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, -- and Kenneth R. Boheler127 -- 14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: -- Strategies and Applications -- Kishore B.S. Pasumarthi and Loren J. Field157 -- 15 Embryonic Stem Cells as a Model for the Physiological Analysis -- of the Cardiovascular System -- Jurgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann, -- Kathrin Banach, Helmut Acker, and Heinrich Sauer 169 -- 16 Isolation of Lineage-Restricted Neural Precursors from Cultured -- ES Cells -- Tahmina Mujtaba and Mahendra S. Rao189 -- 17 Lineage Selection for Generation and Amplification of Neural -- Precursor Cells -- M eng L i2 0 5 -- 18 Selective Neural Induction from ES Cells by Stromal -- Cell-Derived InducingActivity and Its Potential Therapeutic -- Application in Parkinson's Disease -- Hiroshi Kawasaki, Kenji Mizuseki, and Yoshiki Sasai217 -- 19 Epidermal Lineage -- Tammy-Claire Troy and Kursad Turksen229 -- 20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro -- Tammy-Claire Troy and Kursad Turksen255 -- 21 Embryonic Stem Cells as a Model for Studying Melanocyte -- Development -- Toshiyuki Yamane, Shin-lchi Hayashi, and Takahiro Kunisada 261 -- 22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways -- S. Steven Potter, M. Todd Valerius, and Eric W. Brunskill 269 -- 23 ES Cell-Mediated Conditional Transgenesis -- Marina Gertsenstein, Corrinne Lobe and Andras Nagy 285 -- 24 Switching on Lineage Tracers Using Site-Specific Recombination -- Susan M. Dymecki, Carolyn I. Rodriguez, -- and Rajeshwar B. Awatramani309 -- 25 From ES Cells to Mice: The Gene Trap Approach -- Francesco Cecconi and Peter Gruss 335 -- 26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells -- Thomas Floss and Wolfgang Wurst347 -- 27 Phage-Displayed Antibodies to Detect Cell Markers -- Jun Lu and Steven R. Sloan 381 -- 28 Gene Transfer Using Targeted Filamentous Bacteriophage if -- David Larocca, Kristen Jensen-Pergakes, Michael A. Burg, -- and A ndrew Baird 393 -- 29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors -- Jane E. Aubin, Fina Liu, and G. Antonio Candeliere 403 -- 30 Nonradioactive Labeling and Detection of mRNAs Hybridized -- onto Nucleic Acid cDNA Arrays -- Thorsten Hoevel and Manfred Kubbies417 -- 31 Expression Profiling Using Quantitative Hybridization -- on Macroarrays -- Genevieve Pietu and Charles Decraene 425 -- 32 Isolation of Antigen-Specific Intracellular Antibody Fragments -- as Single Chain Fv for Use in Mammalian Cells -- Eric Tse, Grace Chung, and Terence H. Rabbitts433 -- 33 Detection and Visualization of Protein Interactions with Protein -- Fragment Complementation Assays -- Ingrid Remy, Andre Galarneau, and Stephen W. Michnick447 -- 34 Direct Selection of cDNAs by Phage Display -- Reto Crameri, Gernot Achatz, Michael Weichel, -- and Claudio Rhyner461 -- 35 Screening for Protein-Protein Interactions in the Yeast -- Two-Hybrid System -- R. Daniel Gietz and Robin A. Woods471 -- Index 4 87
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1 online resource (xvi, 499 pages)
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illustrations (some color).
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