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The Resource Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis

Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis

Label
Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis
Title
Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis
Creator
Subject
Language
eng
Member of
Cataloging source
MiAaPQ
Literary form
non fiction
Nature of contents
dictionaries
Series statement
Springer Theses Ser
Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis
Label
Development of a Fully Integrated Sample-In-Answer-Out System for Automatic Genetic Analysis
Link
http://libproxy.rpi.edu/login?url=https://ebookcentral.proquest.com/lib/rpi/detail.action?docID=4921428
Publication
Copyright
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Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Dedication -- Supervisoŕs Foreword -- Abstract -- Preface -- Publications -- Acknowledgments -- Contents -- Abbreviations -- Chapter 1: Introduction -- 1.1 Genetic Analysis -- 1.1.1 DNA Extraction -- 1.1.2 DNA Amplification -- 1.1.3 DNA Detection -- 1.1.3.1 Nucleic Acid Electrophoresis -- 1.1.3.2 DNA Microarray -- 1.1.3.3 Quantitative PCR -- 1.1.3.4 Gene Sequencing -- 1.2 Microfluidic -- 1.2.1 Silicon-Based Materials -- 1.2.2 Paper-Based Materials -- 1.2.3 Polymers-Based Material -- 1.3 Microfluidic for Integrated Gene Detection -- 1.3.1 Micrfluidic for DNA Extraction -- 1.3.2 On-Chip Amplification -- 1.3.3 Capillary Electrophoresis Chip -- 1.3.4 Integrated Genetic Analysis System -- 1.4 The Purpose and Significance of This Study -- 1.5 Main Lines of Each Chapter -- References -- Chapter 2: Construction of a General Platform for Capillary Electrophoresis -- 2.1 Chapter Introduction -- 2.2 Construction of Capillary Electrophoresis Instrument -- 2.2.1 Optical System -- 2.2.1.1 DNA Fluorescence Detection -- 2.2.1.2 Optical Design -- 2.2.1.3 Mechanical Design and Adjustment -- 2.2.2 Circuit Board Sets -- 2.2.2.1 Scanning Stage Control -- 2.2.2.2 High-Voltage Module -- 2.2.2.3 Signal Processing Module -- 2.2.3 Chip Loading Stage -- 2.2.4 Industrial Design -- 2.2.5 Software and Operation Process -- 2.2.5.1 Start -- 2.2.5.2 Scan Chip -- 2.2.5.3 Find Channels -- 2.2.5.4 Operation -- 2.2.5.5 Results -- 2.2.5.6 Exit -- 2.3 Capillary Electrophoresis Chip -- 2.3.1 Design of Glass Capillary Electrophoresis Chip -- 2.3.2 Manufacture of Capillary Electrophoresis Chip -- 2.4 Instrument Verification -- 2.4.1 PCR Amplification -- 2.4.2 Pre-treatment of Glass Electrophoresis Chip -- 2.4.3 Test Results -- 2.5 Chapter Summary -- References -- Chapter 3: Integrated Module for Automated DNA Extraction and Amplification -- 3.1 Chapter Introduction
  • 3.2 Integrated Chip for DNA Extraction and Amplification -- 3.2.1 On-Chip Valve Structure -- 3.2.2 Manufacture of DNA Extraction and Amplification Chip -- 3.2.3 Verification on the On-Chip Valve Structure -- 3.3 DNA Extraction and Amplification Equipment -- 3.4 Calibration of the Integrated Sample Preparation System -- 3.4.1 Calibration of PCR Temperature Curve -- 3.4.2 The Automated Extraction and Amplification Process -- 3.5 Verification of Automated DNA Extraction and Amplification System -- 3.6 Screening of Congenital Hearing Loss Mutations Using Automated Sample Preparation System -- 3.6.1 Experimental Setup -- 3.6.2 Experimental Results -- 3.7 Chapter Summary -- References -- Chapter 4: Fully Integrated Genetic Analysis System -- 4.1 Chapter Introduction -- 4.2 Design and Construction of the Fully Integrated Interface -- 4.2.1 Deign of the Interface -- 4.2.2 Design of Sample Electrode -- 4.2.3 Verification of the S Electrode Design -- 4.3 Upgrade of the Fully Integrated Chip -- 4.4 Upgrade of the Integrated Instrument -- 4.4.1 Upgrade of Instrument Design and Structure -- 4.4.2 Upgrade of the Automated Operations -- 4.5 Verification of the Fully Integrated System -- 4.5.1 Verification of the Integrated Interface -- 4.5.2 Fully Integrated Automated Genetic Analysis -- 4.5.3 Limit of Detection of the Automated System -- 4.6 Rapid Pharmacogenetic Typing of Multiple Warfarin-Related Single-Nucleotide Polymorphisms -- 4.6.1 Experimental Setup -- 4.6.2 Experimental Results -- 4.7 Chapter Summary -- References -- Chapter 5: Conclusions and Prospects for Future Work -- 5.1 Conclusions -- 5.2 Prospects for Future Work
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{'f': 'http://opac.lib.rpi.edu/record=b4388618'}
Extent
1 online resource (131 pages)
Form of item
online
Isbn
9789811047534
Media category
computer
Media MARC source
rdamedia
Media type code
c
Sound
unknown sound
Specific material designation
remote

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