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The Resource Dendritic cell protocols, edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight

Dendritic cell protocols, edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight

Label
Dendritic cell protocols
Title
Dendritic cell protocols
Statement of responsibility
edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight
Contributor
Subject
Genre
Language
eng
Summary
It is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics. State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular medicine
Series volume
64
Dendritic cell protocols, edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight
Label
Dendritic cell protocols, edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1385/1592591507
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
pt. 1. Isolation of dendritic cells from animal tissues -- Isolation of dendritic cells from mouse lymph nodes / D. Gabrilovich -- Isolation of mouse spleen dendritic cells / A.J. Stagg -- Isolation of mouse thymic dendritic cells / F. Anjuére -- Isolation of dendritic cells from rat intestinal lymph and spleen / G. MacPherson -- Isolation, enrichment, and culture of murine epidermal Langerhans cells / F. Koch -- Murine intestinal dendritic cell isolation / P. Pavli -- Identification and isolation of rodent lung dendritic cells / P.A. Stumbles -- Isolation and propagation of mouse liver-derived dendritic cells / L. Lu -- pt. 2. Isolation and characterization of human dendritic cells -- Identification and immunophenotypic analyses of a peripheral blood dendritic cells / S.P. Robinson -- Purification of dendritic cells from peripheral blood / S. Patterson -- Isolation of human tonsillar dendritic cells / P.U. Cameron -- Isolation of dendritic cells from human afferent lymph / C.U. Brand -- Isolation of human skin dendritic cells by in vitro migration / C.D. Richters -- Human intestinal dendritic cell isolation / P. Pavli -- Isolation of human lung dendritic cells / M.M. van den Heuvel -- Identification and isolation of synovial dendritic cells / A.R. Pettit -- pt. 3. Derivation of animal dendritic cells from progenitor cells -- Generation of murine bone-marrow-derived dendritic cells / A. Maroof -- Generation of dendritic cells from rat bone marrow / T. Powell -- Derivation of murine dendritic cells from thymic precursors / K. Lucas -- pt. 4. Generation of murine dendritic cell lines -- Generation of mouse dendritic cell lines / S. Citterio -- Generation of Langerhans cell-like dendritic cells from murine fetal skin / T. Jakob -- Derivation of dendritic cell lines from mouse skin / A. Takashima -- pt. 5. Generation of human dendritic cells from progenitor cells -- Propagation of human dendritic cells in vitro / C. Caux -- Generation of dendritic cells from lymphoid precursors / A. Galy -- Dendritic cell generation from highly purified CD14+ monocytes / W.F. Pickl -- Generation of blood-derived human dendritic cells for antitumor immunotherapy / M.F. Gilliet -- pt. 6. Aspects of dendritic cell function -- Chemotaxis of in vitro cultured human dendritic cells / W. Luini -- Adhesion of dendritic cells to endothelia / K.A. Brown -- Transendotherlial migration and reverse transmigration of in vitro cultured human dendritic cells / G. Bianchi -- In vivo assays of Langerhans cells migration / M. Cumberbatch -- In vitro migration of rat dendritic cells / K. Matsuno -- Antigen uptake by dendritic cells / C. Caux -- Uptake of antigen-antibody complexes by human dendritic cells / N.A. Fanger -- Immunoelectron microscopy of antigen processing in dendritic cells / M.J. Kleijmeer -- Purification and characterization of MHC class II containing organelles in mouse bone-marrow-derived dendritic cells / P. Pierre -- Detection of intracellular cytokines in dendritic cells / P. Kelleher -- Adenoviral-vector-mediated gene transfer to dendritic cells / W. Song -- Stimulation of anti-HIV-1 cytotoxic T lymphocytes by dendritic cells / X. Huang
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https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781592591503&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3356776'}
Extent
1 online resource (xvii, 470 pages)
Form of item
online
Isbn
9781592591503
Lccn
00047235
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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