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The Resource DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke

DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke

DNA topoisomerases : methods and protocols
DNA topoisomerases
Title remainder
methods and protocols
Statement of responsibility
edited by Duncan J. Clarke
"Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls." "Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field."--Jacket
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Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
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  • Springer protocols
  • Methods in molecular biology,
Series volume
DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke
DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke
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Bibliography note
Includes bibliographical references and index
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online resource
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Emerging themes in DNA topoisomerase research / Duncan J. Clarke and Andrew Lane -- Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis / Stephen D. Levene -- Two-dimensional ararose gel electrophoresis of DNA topoisomers / Joaquim Roca -- Cleavage of plasmid DNA by eukaryotic topoisomerase II / Omari J. Bandele, Neil Osheroff -- Assays for the preferential binding of human topoisomerase I to supercoiled DNA / Zheng Yang and James J. Champoux -- Monitoring the topoisomerase II DNA gate conformational change with fluorescence resonance energy transfer / Tammy R.L. Collins and Tao-shih Hsieh -- Single-molecule magnetic tweezers studies of type IB topoisomerases / Jan Lipfert, Daniel A. Koster, Igor D. Vilfan, Susanne Hage and Nynke H. Dekker -- Dissolution of double holliday junctions by the concerted action of BLM and topoisomerase III / Csanad Z. Bachrati and Ian D. Hickson -- ChIP-on-Chip analysis of DNA topoisomerases / Rodrigo Bermejo, Yuki-Mori Katou, Katsuhiko Shirahige and Marco Foiani -- Analyzing Top2 distribution on yeast chromosomes by chromatin immunoprecipitation / Melissa Baldwin, Tariq Warsi and Jeff Bachant -- Binding of DNA topoisomerases I and II to replication origins / Arturo Falaschi -- Cytometric assessment of DNA damage induced by DNA topoisomerase inhibitors / Zbigniew Darzynikiewicz, Dorota H. Halicka and Toshiki Tanaka -- Analysis of the topoisomerase II-dependent decatenation G2 checkpoint and checkpoint kinases in human cells / William K. Kaufmann -- Assaying topoisomerase II checkpoints in yeast / Katherine Furniss, Amit C.J. Vas, Anderw Lane and Duncan J. Clarke -- Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction / Juan F. Gimenez-Abian and Duncan J. Clarke -- Top2 SUMO conjugation in yeast cell lysates / Melissa Baldwin and Jeff Bachant -- Analysis of SUMOylation of topoisomerase II in living cells / John R. Daum, Yin Yuan Mo and Gary J. Gorbsky -- Depletion and mutation of topoisomerase II in animal cells / Andrew C.G. Porter
{'f': ''}
1 online resource (x, 267 pages)
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illustrations (some color).
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Electronic reproduction.
unknown sound
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Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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