Coverart for item
The Resource DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke

DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke

Label
DNA topoisomerases : methods and protocols
Title
DNA topoisomerases
Title remainder
methods and protocols
Statement of responsibility
edited by Duncan J. Clarke
Contributor
Subject
Language
eng
Summary
"Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls." "Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field."--Jacket
Member of
Cataloging source
CUS
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
582
DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke
Label
DNA topoisomerases : methods and protocols, edited by Duncan J. Clarke
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-60761-340-4
Publication
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Related Location
Related Agents
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Emerging themes in DNA topoisomerase research / Duncan J. Clarke and Andrew Lane -- Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis / Stephen D. Levene -- Two-dimensional ararose gel electrophoresis of DNA topoisomers / Joaquim Roca -- Cleavage of plasmid DNA by eukaryotic topoisomerase II / Omari J. Bandele, Neil Osheroff -- Assays for the preferential binding of human topoisomerase I to supercoiled DNA / Zheng Yang and James J. Champoux -- Monitoring the topoisomerase II DNA gate conformational change with fluorescence resonance energy transfer / Tammy R.L. Collins and Tao-shih Hsieh -- Single-molecule magnetic tweezers studies of type IB topoisomerases / Jan Lipfert, Daniel A. Koster, Igor D. Vilfan, Susanne Hage and Nynke H. Dekker -- Dissolution of double holliday junctions by the concerted action of BLM and topoisomerase III / Csanad Z. Bachrati and Ian D. Hickson -- ChIP-on-Chip analysis of DNA topoisomerases / Rodrigo Bermejo, Yuki-Mori Katou, Katsuhiko Shirahige and Marco Foiani -- Analyzing Top2 distribution on yeast chromosomes by chromatin immunoprecipitation / Melissa Baldwin, Tariq Warsi and Jeff Bachant -- Binding of DNA topoisomerases I and II to replication origins / Arturo Falaschi -- Cytometric assessment of DNA damage induced by DNA topoisomerase inhibitors / Zbigniew Darzynikiewicz, Dorota H. Halicka and Toshiki Tanaka -- Analysis of the topoisomerase II-dependent decatenation G2 checkpoint and checkpoint kinases in human cells / William K. Kaufmann -- Assaying topoisomerase II checkpoints in yeast / Katherine Furniss, Amit C.J. Vas, Anderw Lane and Duncan J. Clarke -- Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction / Juan F. Gimenez-Abian and Duncan J. Clarke -- Top2 SUMO conjugation in yeast cell lysates / Melissa Baldwin and Jeff Bachant -- Analysis of SUMOylation of topoisomerase II in living cells / John R. Daum, Yin Yuan Mo and Gary J. Gorbsky -- Depletion and mutation of topoisomerase II in animal cells / Andrew C.G. Porter
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781607613404&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357401'}
Extent
1 online resource (x, 267 pages)
Form of item
online
Isbn
9781607613404
Lccn
2009933621
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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