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The Resource DNA-protein interactions : principles and protocols, edited by Benoit P. Leblanc and Sebastien Rodrigue

DNA-protein interactions : principles and protocols, edited by Benoit P. Leblanc and Sebastien Rodrigue

Label
DNA-protein interactions : principles and protocols
Title
DNA-protein interactions
Title remainder
principles and protocols
Statement of responsibility
edited by Benoit P. Leblanc and Sebastien Rodrigue
Contributor
Editor
Subject
Genre
Language
eng
Summary
This title presents the most recent technical advances in DNA-protein interaction research. With fully updated chapters that describe techniques proven by their continuous value, it also includes new chapters dealing with larger-scale experiments, to reflect recent advances in 'big biology'. As a whole, it offers a very useful compendium of protocols, allowing readers to delve into the intricacies of protein-DNA interaction at levels ranging from the very small to the very complex
Member of
Cataloging source
RML
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
  • Methods in molecular biology,
  • Springer protocols
Series volume
volume 1334
DNA-protein interactions : principles and protocols, edited by Benoit P. Leblanc and Sebastien Rodrigue
Label
DNA-protein interactions : principles and protocols, edited by Benoit P. Leblanc and Sebastien Rodrigue
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-4939-2877-4
Publication
Copyright
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Related Location
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Precise identification of genome-wide transcription start sites in bacteria by 5'-rapid amplification of cDNA ends (5'-RACE)
  • Analysis of DNA supercoiling induced by DNA-protein interactions
  • Precise identification of DNA-binding proteins genomic location by exonuclease coupled chromatin immunoprecipitation (ChIP-exo)
  • The cruciform DNA mobility shift assay: A tool to study proteins that recognize bent DNA
  • Individual and sequential chromatin immunoprecipitation protocols
  • Chromatin endogenous cleavage (ChEC) as a method to quantify protein interaction with genomic DNA in saccharomyces cerevisiae
  • Selection and validation of spacer sequences for CRISPR-Cas9 genome editing and transcription regulation in bacteria
  • Detection of short-range DNA interactions in mammalian cells using high-resolution circular chromosome conformation capture coupled to deep sequencing
  • Global mapping of open chromatin regulatory elements by formaldehyde-assisted isolation of regulatory elements followed by sequencing (FAIRE-seq)
  • Aggregate and heatmap representations of genome-wide localization data using VAP, a versatile aggregate profiler
  • Electrophoretic mobility shift assay using radiolabeled DNA probes
  • Circular dichroism for the analysis of protein-DNA interactions
  • Quantitative investigation of protein-nucleic acid interactions by biosensor surface plasmon resonance
  • Identification of nucleic acid high affinity binding sequences of proteins by SELEX
  • In vitro DNase i footprinting
  • Determining the architecture of a protein-DNA complex by combining FeBABE cleavage analyses, 3-D printed structures, and the ICM molsoft program
  • In cellulo DNA analysis: LMPCR footprinting
  • Southwestern blotting assay
  • Single-molecule approaches for the characterization of riboswitch folding mechanisms
  • Probing of nascent riboswitch transcripts
  • Functional studies of DNA-protein interactions using fret techniques
http://library.link/vocab/cover_art
https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781493928774&userID=ebsco-test&password=ebsco-test
Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3753111'}
Edition
Fourth edition.
Extent
1 online resource (xvi, 347 pages)
Form of item
online
Isbn
9781493928774
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Sound
unknown sound
Specific material designation
remote

Library Locations

    • Folsom LibraryBorrow it
      110 8th St, Troy, NY, 12180, US
      42.729766 -73.682577
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