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The Resource Cardiac gene expression : methods and protocols, edited by Jun Zhang and Gregg Rokosh

Cardiac gene expression : methods and protocols, edited by Jun Zhang and Gregg Rokosh

Label
Cardiac gene expression : methods and protocols
Title
Cardiac gene expression
Title remainder
methods and protocols
Statement of responsibility
edited by Jun Zhang and Gregg Rokosh
Contributor
Subject
Genre
Language
eng
Summary
Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion
Member of
Cataloging source
COO
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
366
Cardiac gene expression : methods and protocols, edited by Jun Zhang and Gregg Rokosh
Label
Cardiac gene expression : methods and protocols, edited by Jun Zhang and Gregg Rokosh
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-59745-030-0
Publication
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Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Cardiac gene expression: the global perspective. Microarray analysis of gene expression in murine cardiac graft infiltrating cells / Yurong Liang, Xin Lu, and David L. Perkins. Expression profiling using Affymetrix GeneChip probe arrays / Martina Schinke-Braun and Jennifer A. Couget. Serial analysis of gene expression (SAGE): a useful tool to analyze the cardiac transcriptome / Kirill V. Tarasov [and others] Functional genomics by cDNA subtractive hybridization / Christophe Depre. Statistical methods in cardiac gene expression profiling: from image to function / Sek Won Kong -- Cardiac gene regulation: gene-specific mRNA measurement in the myocardium. Measurement of cardiac gene expression by reverse transcription polymerase chain reaction (RT-PCR) / Nicola King. Quantitative (real-time) RT-PCR in cardiovascular research / Kevin John Ashton and John Patrick Headrick. RNase protection assay for quantifying gene expression levels / Yongxia Qu and Mohamed Boutjdir. In situ hybridization: a technique to study localization of cardiac gene expression / Thierry P. Calmels and David Mazurais -- Cardiac gene regulation: promoter characterization in the myocardium. Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay / Jim Jung-Ching Lin [and others] -- Mapping transcriptional start sites and in silico DNA footprinting / Martin E. Cullen and Paul J.R. Barton. Characterization of cardiac gene promoter activity: reporter constructs and heterologous promoter studies / Hsiao-Huei Chen and Alexandre F.R. Stewart -- In silico assessment of regulatory cis-elements and gene regulation. Comparative genomics: a tool to functionally annotate human DNA / Jan-Fang Cheng, James R. Priest, and Len A. Pennacchio. Developing computational resources in cardiac gene expression / Michael B. Bober and Raimond Winslow -- Cardiac single nucleotide polymorphisms. In silico analysis of SNPs and other high-throughput data / Neema Jamshidi, Thuy D. Vo, and Bernhard O. Palsson. Discovery and identification of sequence polymorphisms and mutations with MALDI-TOF MS / Dirk van den Boom and Mathias Ehrich -- Gene overexpression and targeting in the myocardium. Conditional targeting: inducible deletion by cre recombinase / Kelly R. O'Neal and Ramtin Agah. Cardiomyocyte preparation, culture, and gene transfer / Alexander H. Maass and Massimo Buvoli. Adeno-associated viral vector-delivered hypoxia-inducible gene expression in ischemic hearts / Hua Su and Yuet Wai Kan. Lentivirus-mediated gene expression / Jing Zhao and Andrew M.L. Lever
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Dimensions
unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357042'}
Extent
1 online resource (xi, 365 pages)
Form of item
online
Isbn
9781597450300
Lccn
2006008866
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Specific material designation
remote

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