Coverart for item
The Resource Carbohydrate microarrays : methods and protocols, edited by Yann Chevolot

Carbohydrate microarrays : methods and protocols, edited by Yann Chevolot

Label
Carbohydrate microarrays : methods and protocols
Title
Carbohydrate microarrays
Title remainder
methods and protocols
Statement of responsibility
edited by Yann Chevolot
Contributor
Subject
Genre
Language
eng
Summary
Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe
Member of
Cataloging source
GW5XE
Illustrations
illustrations
Index
index present
Language note
English
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology,
Series volume
808
Carbohydrate microarrays : methods and protocols, edited by Yann Chevolot
Label
Carbohydrate microarrays : methods and protocols, edited by Yann Chevolot
Link
http://libproxy.rpi.edu/login?url=http://link.springer.com/10.1007/978-1-61779-373-8
Publication
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Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Recent advances and future challenges in glycan microarray technology -- Chemical synthesis of carbohydrates and their surface immobilization: A brief introduction -- General consideration on sialic acid chemistry -- Synthesis of azido-functionalized carbohydrates for the design of glycoconjugates -- Polypyrrole-oligosaccharide microarray for the measurement of biomolecular interactions by surface plasmon resonance imaging -- Glycosylated self-assembled monolayers for arrays and surface analysis -- Carbohydrate microarrays for enzymatic reactions and quantification of binding affinities for glycan-protein interactions -- Neoglycolipid-based oligosaccharide microarray system: Preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses -- Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and application in ligand identification of P-type lectins -- Production of fluorous-based microarrays with uncharged carbohydrates -- General procedure for the synthesis of neoglycoproteins and immobilization on epoxide-modified glass slides -- Immobilization of polyacrylamide-based glycoconjugates on solid phase in immunosorbent assays -- Surface plasmon resonance imaging analysis of protein binding to a sialoside-based carbohydrate microarray -- Glycoarray by DNA-directed immobilization -- Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides -- Polysaccharide microarrays: Application to the identification of heparan sulphate mimetics -- Carbohydrate antigen microarrays -- Probing virus-glycan interactions using glycan microarrays -- MALDI-ToF MS analysis of glycosyltransferase activities on gold surface arrays -- Microarray technology using glycans extracted from natural sources for serum antibody fluorescent detection -- Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray -- Microarray method for the rapid detection of glycosaminoglycan-protein interactions -- Neoglycolipid-based "Designer" Oligosaccharide microarrays to define beta-glucan ligands for Dectin-1 -- Measurement of antibodies to pneumococcal polysaccharides with luminex xmap microsphere-based liquid arrays -- Carbohydrate microarrays in 96-well polystyrene microtiter plates -- Photoimmobilization of saccharides -- Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate -- Combinatorial glycoarray
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https://contentcafe2.btol.com/ContentCafe/Jacket.aspx?Return=1&Type=S&Value=9781617793738&userID=ebsco-test&password=ebsco-test
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unknown
http://library.link/vocab/discovery_link
{'f': 'http://opac.lib.rpi.edu/record=b3357699'}
Extent
1 online resource (xv, 427 pages)
File format
unknown
Form of item
online
Isbn
9781617793738
Lccn
2011938687
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote

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